DynExpModule::Widefield::WidefieldMicroscope Class Reference

#include <WidefieldMicroscope.h>

Inheritance diagram for DynExpModule::Widefield::WidefieldMicroscope:

Classes
struct	ConfocalOptimizationStateType

Public Types
using	ParamsType = WidefieldMicroscopeParams
using	ConfigType = WidefieldMicroscopeConfigurator
using	ModuleDataType = WidefieldMicroscopeData
Public Types inherited from DynExp::QModuleBase
using	ParamsType = QModuleParamsBase
	Type of the parameter class belonging to this Object type. Declare this alias in every derived class with the respective parameter class accompanying the derived Object. "
using	ConfigType = QModuleConfiguratorBase
	Type of the configurator class belonging to this Object type. Declare this alias in every derived class with the respective configurator class accompanying the derived Object.
using	ModuleDataType = QModuleDataBase
	Type of the data class belonging to this ModuleBase type. Declare this alias in every derived class with the respective data class accompanying the derived ModuleBase.
Public Types inherited from DynExp::ModuleBase
using	ParamsType = ModuleParamsBase
	Type of the parameter class belonging to this Object type. Declare this alias in every derived class with the respective parameter class accompanying the derived Object. "
using	ConfigType = ModuleConfiguratorBase
	Type of the configurator class belonging to this Object type. Declare this alias in every derived class with the respective configurator class accompanying the derived Object.
using	ModuleDataType = ModuleDataBase
	Type of the data class belonging to this ModuleBase type. Declare this alias in every derived class with the respective data class accompanying the derived ModuleBase.
using	ModuleDataTypeSyncPtrType = Util::SynchronizedPointer< ModuleDataType >
	Alias for the return type of ModuleBase::GetModuleData(). Data class instances wrapped into Util::SynchronizedPointer can be accessed in a thread-safe way.
using	ModuleDataTypeSyncPtrConstType = Util::SynchronizedPointer< const ModuleDataType >
	Alias for the return type of ModuleBase::GetModuleData() const. Data class instances wrapped into Util::SynchronizedPointer can be accessed in a thread-safe way.
using	ModuleDataGetterType = Util::CallableMemberWrapper< ModuleBase, ModuleDataTypeSyncPtrType(ModuleBase::*)(const std::chrono::milliseconds)>
	Invoking an instance of this alias is supposed to call ModuleBase::GetModuleData() of the instance the Util::CallableMemberWrapper has been constructed with.
Public Types inherited from DynExp::RunnableObject
using	ParamsType = RunnableObjectParams
	Type of the parameter class belonging to this Object type. Declare this alias in every derived class with the respective parameter class accompanying the derived Object. "
using	ConfigType = RunnableObjectConfigurator
	Type of the configurator class belonging to this Object type. Declare this alias in every derived class with the respective configurator class accompanying the derived Object.
Public Types inherited from DynExp::Object
using	ParamsType = ParamsBase
	Type of the parameter class belonging to this Object type. Declare this alias in every derived class with the respective parameter class accompanying the derived Object.
using	ParamsTypeSyncPtrType = Util::SynchronizedPointer< ParamsType >
	Alias for the return type of Object::GetParams(). Parameters wrapped into Util::SynchronizedPointer can be accessed in a thread-safe way.
using	ParamsConstTypeSyncPtrType = Util::SynchronizedPointer< const ParamsType >
	Alias for the return type of Object::GetParams() const. Parameters wrapped into Util::SynchronizedPointer can be accessed in a thread-safe way.
using	ConfigType = ConfiguratorBase
	Type of the configurator class belonging to this Object type. Declare this alias in every derived class with the respective configurator class accompanying the derived Object.
using	ParamsGetterType = Util::CallableMemberWrapper< Object, decltype(&Object::GetParams)>
	Invoking an instance of this alias is supposed to call Object::GetParams() of the instance the Util::CallableMemberWrapper has been constructed with.

Public Member Functions
	WidefieldMicroscope (const std::thread::id OwnerThreadID, DynExp::ParamsBasePtrType &&Params)
virtual	~WidefieldMicroscope ()
virtual std::string	GetName () const override
	Returns the name of this Object type.
virtual std::string	GetCategory () const override
	Returns the category of this Object type.
std::chrono::milliseconds	GetMainLoopDelay () const override final
	Specifies in which time intervals the module's event queue runs to handle pending events.
void	RegisterConfocalGraphEvents (DynExpQuick::DynExpSurfaceGraphBackend *ConfocalGraph)
void	OnSaveCurrentImage (DynExp::ModuleInstance *Instance, QString Filename) const
void	OnGoToSamplePos (DynExp::ModuleInstance *Instance, QPointF SamplePos) const
void	OnBringMarkerToConfocalSpot (DynExp::ModuleInstance *Instance, QPoint MarkerPos, QPointF SamplePos) const
void	OnRunCharacterizationFromID (DynExp::ModuleInstance *Instance, Util::MarkerGraphicsView::MarkerType::IDType ID) const
Public Member Functions inherited from DynExp::QModuleBase
	QModuleBase (const std::thread::id OwnerThreadID, DynExp::ParamsBasePtrType &&Params)
	Constructs a QModuleBase instance.
virtual	~QModuleBase ()=0
bool	HasUI () const noexcept override final
	Determines whether this module possesses a user interface (UI) which is shown in a window dedicated to the module.
QAction &	InitUI (DynExpManager &DynExpMgr, QMdiArea *const MdiArea)
	Sets up the module's user interface widgets and window frames. Called by the main user interface thread in DynExpManager::RegisterModuleUI().
void	HideUI ()
	Removes MdiSubWindow, DockWidget, and ModuleWindowFocusAction setting them to nullptr.
virtual void	DisableUI ()
	Disables all user interface controls in Widget. Does nothing if Widget is nullptr.
virtual void	UpdateUI ()
	Enables the user interface controls in Widget. Does nothing if Widget is nullptr. Calls UpdateUIChild().
void	UpdateModuleWindowFocusAction ()
	Updates the icon assigned to ModuleWindowFocusAction depending on whether Widget is docked to or undocked from MdiArea. Called by the main user interface thread in DynExpManager::UpdateModuleWindowsActionIcons().
void	DockWindow () noexcept
	Docks Widget to MdiArea. Does nothing if any of Widget, MdiArea, MdiSubWindow, or DockWidget are nullptr.
void	UndockWindow () noexcept
	Undocks Widget from MdiArea. Does nothing if any of Widget, MdiArea, MdiSubWindow, or DockWidget are nullptr.
void	DockUndockWindow () noexcept
	Toggles the docking state of Widget. Does nothing if any of Widget, MdiArea, MdiSubWindow, or DockWidget are nullptr.
void	SetFocus () noexcept
	Focuses/activates Widget and moves it on top of other windows if possible. Does nothing if any of Widget, MdiArea, MdiSubWindow, or DockWidget are nullptr.
void	FocusMainWindow () noexcept
	Focuses/activates DynExp's main window calling DynExpManager::FocusMainWindow(). Does nothing if Widget is nullptr.
void	SendKeyPressEventToMainWindow (QKeyEvent *Event) noexcept
	Sends a Qt key press event to DynExp's main window calling DynExpManager::PostKeyPressEvent(). Does nothing if Widget is nullptr.
bool	IsWindowDocked () noexcept
	Checks whether Widget is docked.
bool	IsActiveWindow ()
	Checks whether Widget is docked and active.
Public Member Functions inherited from DynExp::ModuleBase
	ModuleBase (const std::thread::id OwnerThreadID, ParamsBasePtrType &&Params)
	Constructs a ModuleBase instance.
virtual	~ModuleBase ()=0
void	RestoreWindowStatesFromParams ()
	RestoreWindowStatesFromParams() only calls RestoreWindowStatesFromParamsChild(). Override RestoreWindowStatesFromParamsChild() to restore the styles of possibly owned windows according to the style saved in the module's parameters.
void	UpdateParamsFromWindowStates ()
	UpdateParamsFromWindowStates() only calls UpdateParamsFromWindowStatesChild(). Override UpdateParamsFromWindowStatesChild() to store the styles of possibly owned windows in the module's parameters.
ModuleDataTypeSyncPtrType	GetModuleData (const std::chrono::milliseconds Timeout=GetModuleDataTimeoutDefault)
	Locks the mutex of the module data class instance ModuleData assigned to this ModuleBase instance and returns a pointer to the locked ModuleData. Module data should not be locked by having called this function while subsequently calling a derived module's method which also makes use of the module's data by locking it. If this happens, the module data's mutex is locked recursively. In principle, this does no harm since Util::ISynchronizedPointerLockable supports that. But, it is not considered good practice.
ModuleDataTypeSyncPtrConstType	GetModuleData (const std::chrono::milliseconds Timeout=GetModuleDataTimeoutDefault) const
	Locks the mutex of the module data class instance ModuleData assigned to this ModuleBase instance and returns a pointer to the locked ModuleData. Module data should not be locked by having called this function while subsequently calling a derived module's method which also makes use of the module's data by locking it. If this happens, the module data's mutex is locked recursively. In principle, this does no harm since Util::ISynchronizedPointerLockable supports that. But, it is not considered good practice.
void	EnqueueEvent (ModuleDataBase::EventPtrType &&Event) const
	Enqueues Event at the module event queue's back. Takes ownership of the event. Notifies the module owning the respective ModuleDataBase's instance that a new event has been enqueued.
template<typename ReceiverType , typename EventType , typename... ArgsTs>
void	MakeAndEnqueueEvent (ReceiverType *Receiver, EventType EventFuncPtr, ArgsTs &&...Args) const
	Calls MakeEvent() to construct a new event and subsequently enqueues the event into the module's event queue. Logical const-ness: this is a const member function to allow pointers to const ModuleBase inserting events into the module's event queue. These kind of pointers are e.g. returned by RunnableInstance::GetOwner() which can be called by events' EventBase::InvokeChild() functions. For const ModuleBase*, it is possible to insert events into the event queue, but not to change the ModuleBase object itself (e.g. calling Object::Reset()).
virtual bool	TreatModuleExceptionsAsWarnings () const
	Determines whether this module should be terminated if an exception leaves the module's main loop or respective event handlers.
Public Member Functions inherited from DynExp::RunnableObject
	RunnableObject (const std::thread::id OwnerThreadID, ParamsBasePtrType &&Params)
	Constructs an Object instance.
virtual	~RunnableObject ()=0
void	SetPaused (bool Pause, std::string Description="")
	Pauses or resumes a RunnableObject instance. Its thread stays running, but the instance does not perform any action while it is paused.
bool	IsRunning () const noexcept
	Returns Running.
bool	IsPaused () const noexcept
	Returns Paused.
bool	IsExiting () const noexcept
	Returns ShouldExit.
auto	GetStartupType () const noexcept
	Returns Startup.
auto	GetReasonWhyPaused () const
	Returns ReasonWhyPaused.
bool	Run (QWidget *ParentWidget=nullptr)
	Starts the RunnableObject instance's thread and ensures that all Object instances linked to this instance via ObjectLink parameters are in a ready state. Calls RunChild() which is to be overridden by derived classes to start a thread and to pass this thread back by calling StoreThread().
bool	RunIfRunAutomatic ()
	Calls Run() if RunnableObjectParams::Startup is set to RunnableObjectParams::Automatic.
bool	RunIfRunOnCreation ()
	Calls Run() if RunnableObjectParams::Startup is set to RunnableObjectParams::OnCreation.
void	Terminate (bool Force=false, const std::chrono::milliseconds Timeout=TerminateTimeoutDefault)
	Notifies the RunnableObject instance's thread to terminate and waits until it has ended. Calls TerminateImpl().
Public Member Functions inherited from Util::INonCopyable
	INonCopyable (const INonCopyable &)=delete
INonCopyable &	operator= (const INonCopyable &)=delete
Public Member Functions inherited from DynExp::Object
ItemIDType	GetID () const noexcept
	Returns the ID of this Object instance. Thread-safe since ID is const.
std::string	GetCategoryAndName () const
	Builds a string from an Object's category and name to allow the user to identify an Object's type.
void	EnsureReadyState (bool IsAutomaticStartup)
	Ensures that this Object instance is ready by possibly starting its worker thread or by opening connections to hardware devices.
void	CheckLinkedObjectStates () const
	Checks whether Object instances this instance uses are in a ready state. Override CheckLinkedObjectStatesChild() to implement this behavior.
void	Reset ()
	Resets this Object instance (including all its derived classes) by calling ResetImpl(). A reset implies that the derived classes reload all their parameters from Params and that all LinkedObjectWrapper instances owned by this Object instance become invalidated.
void	BlockIfUnused (const std::chrono::milliseconds Timeout=Util::ILockable::DefaultTimeout)
	Blocks this Object instance setting Object::IsBlocked to true. Refer to Object::IsBlocked for the consequences.
ParamsConstTypeSyncPtrType	GetParams (const std::chrono::milliseconds Timeout=GetParamsTimeoutDefault) const
	Locks the mutex of the parameter class instance Params assigned to this Object instance and returns a pointer to the locked Params.
ParamsTypeSyncPtrType	GetParams (const std::chrono::milliseconds Timeout=GetParamsTimeoutDefault)
	Locks the mutex of the parameter class instance Params assigned to this Object instance and returns a pointer to the locked Params.
auto	GetObjectName (const std::chrono::milliseconds Timeout=GetParamsTimeoutDefault) const
	Returns the name of this Object instance.
bool	IsSharedUsageEnabled (const std::chrono::milliseconds Timeout=GetParamsTimeoutDefault) const
	Returns whether shared usage has been enabled for this Object instance. Refer to ParamsBase::UsageType.
void	SetWarning (std::string Description, int ErrorCode) const
	Setter for Object::Warning. Sets the warning by a description and an error code.
void	SetWarning (const Util::Exception &e) const
	Setter for Object::Warning. Sets the warning by retrieving the warning data from an exception e.
void	ClearWarning () const
	Resets Object::Warning.
auto	GetWarning () const
	Returns Object::Warning in a thread-safe way by copying its internal data.
std::exception_ptr	GetException (const std::chrono::milliseconds Timeout=Util::ILockable::DefaultTimeout) const
	Returns a pointer to the exception which has caused this Object instance to fail.
bool	IsReady () const
	Returns wheter this Object instance is ready (e.g. it is running or connected to a hardware device) and not blocked (refer to Object::IsBlocked).
auto	GetUseCount (const std::chrono::milliseconds Timeout=Util::ILockable::DefaultTimeout) const
	Counts the registered useres in a thread-safe way.
bool	IsUnused (const std::chrono::milliseconds Timeout=Util::ILockable::DefaultTimeout) const
	Returns whether this Object instance is used by other instances.
auto	GetUserIDs (const std::chrono::milliseconds Timeout=Util::ILockable::DefaultTimeout) const
	Returns a list of the IDs of the registered users in a thread-safe way.
auto	GetUserNamesString (const std::chrono::milliseconds Timeout=Util::ILockable::DefaultTimeout) const
	Builds a string describing which users are registered containing their object names, categories and type names in a thread-safe way.

Static Public Member Functions
static constexpr auto	Name () noexcept
static constexpr auto	Category () noexcept
Static Public Member Functions inherited from DynExp::ModuleBase
static constexpr auto	Category () noexcept
	Every derived class has to redefine this function.
Static Public Member Functions inherited from DynExp::Object
static std::string	CategoryAndNameToStr (const std::string &Category, const std::string &Name)
	Builds a string from an Object's category and name to allow the user to identify an Object's type.
static std::string	RemoveCategoryAndName (const std::string &ObjectDesc)
	Removes the category and name part built by Object::CategoryAndNameToStr() from the string ObjectDesc constructed by LinkedObjectWrapper::GetLinkedObjectDesc().

Private Types
enum class	PositionerStateType { WaitingForMovement , Moving , Arrived }
enum class	WidefieldImageProcessingStateType { Waiting , Finished , Failed }
enum class	ConfocalOptimizationThreadReturnType { NextStep , Finished , Failed }
using	AtomicPositionerStateType = std::atomic< PositionerStateType >
using	AtomicWidefieldImageProcessingStateType = std::atomic< WidefieldImageProcessingStateType >
using	ConfocalOptimizationFeedbackType = double

Private Member Functions
Util::DynExpErrorCodes::DynExpErrorCodes	ModuleMainLoop (DynExp::ModuleInstance &Instance) override final
	Module main loop. The function is executed periodically by the module thread. Also refer to GetMainLoopDelay().
void	ResetImpl (dispatch_tag< QModuleBase >) override final
std::unique_ptr< DynExp::QModuleWidget >	MakeUIWidget () override final
	Used by InitUI() as a factory function for the module's user interface widget. Create the widget here using std::make_unique and then use Connect() to connect Qt signals to the module's event functions.
void	UpdateUIChild (const ModuleBase::ModuleDataGetterType &ModuleDataGetter) override final
bool	IsReadyState () const noexcept
StateType	ResetState (Util::SynchronizedPointer< ModuleDataType > &ModuleData) const
	Aborts a measurement or experimental sequence and continues with the ready state.
bool	IsCharacterizingSample () const noexcept
	Returns true if an entire sample (multiple cells) are processed, false if only a single cell is processed.
void	MoveSampleTo (const ModuleDataType::PositionPoint &Point, Util::SynchronizedPointer< ModuleDataType > &ModuleData) const
bool	IsSampleMoving (Util::SynchronizedPointer< ModuleDataType > &ModuleData) const
	Checks whether the sample is moving in x- or y-direction. The z-direction is ignored currently since this module doesn't currently make use of it.
void	SetFocus (Util::SynchronizedPointer< ModuleDataType > &ModuleData, double Voltage, bool IgnoreOffset=false) const
ModuleDataType::PositionPoint	CalcMarkerToConfocalSpotDestiny (Util::SynchronizedPointer< const ParamsType > &ModuleParams, Util::SynchronizedPointer< ModuleDataType > &ModuleData, QPoint MarkerPos, QPointF SamplePos) const
void	BringMarkerToConfocalSpot (Util::SynchronizedPointer< const ParamsType > &ModuleParams, Util::SynchronizedPointer< ModuleDataType > &ModuleData, QPoint MarkerPos, QPointF SamplePos) const
QSurfaceDataArray	CalculateConfocalScanPositions (const int Width, const int Height, const int DistPerPixel, const WidefieldMicroscopeData::PositionPoint CenterPosition) const
void	UpdatePumpPower (Util::SynchronizedPointer< ModuleDataType > &ModuleData) const
void	PrepareImageRecording (Util::SynchronizedPointer< ModuleDataType > &ModuleData) const
void	RecordImage (Util::SynchronizedPointer< ModuleDataType > &ModuleData) const
StateType	InitiateReadCellIDFromImage (Util::SynchronizedPointer< ModuleDataType > &ModuleData) const
StateType	InitiateLocalizationFromImage (Util::SynchronizedPointer< ModuleDataType > &ModuleData) const
void	PrepareAPDsForConfocalMode (Util::SynchronizedPointer< ModuleDataType > &ModuleData) const
void	InitializeConfocalOptimizer (Util::SynchronizedPointer< ModuleDataType > &ModuleData) const
void	SetHBTSwitch (Util::SynchronizedPointer< const ParamsType > &ModuleParams, Util::SynchronizedPointer< ModuleDataType > &ModuleData, bool IsHBTMode) const
void	InitializeHBT (Util::SynchronizedPointer< ModuleDataType > &ModuleData) const
void	StopHBT (Util::SynchronizedPointer< ModuleDataType > &ModuleData) const
StateType	StartAutofocus (Util::SynchronizedPointer< ModuleDataType > &ModuleData) const
StateType	StartAutoMeasureLocalization (Util::SynchronizedPointer< ModuleDataType > &ModuleData) const
StateType	StartAutoMeasureCharacterization (Util::SynchronizedPointer< ModuleDataType > &ModuleData, Util::MarkerGraphicsView::MarkerType::IDType FirstEmitterID=-1) const
StateType	StartAutoMeasureSampleCharacterization (Util::SynchronizedPointer< ModuleDataType > &ModuleData) const
std::filesystem::path	BuildFilename (Util::SynchronizedPointer< ModuleDataType > &ModuleData, std::string_view FilenameSuffix) const
ModuleDataType::PositionPoint	RandomPointInCircle (ModuleDataType::PositionType Radius) const
	Returns a uniformly-distributed random coordinate within a circle of radius Radius, not thread-safe.
void	ConfocalOptimizationInitPromises ()
double	ConfocalOptimizationFunc (const gsl_vector *vector) noexcept
void	OnInit (DynExp::ModuleInstance *Instance) const override final
	This event is triggered right before the module thread starts. Override it to lock instruments this module depends on (via RunnableInstance::LockObject()) and to register/subscribe for events (via InterModuleEvent::Register()).
void	OnExit (DynExp::ModuleInstance *Instance) const override final
	This event is triggered right before the module thread terminates (not due to an exception, in this case refer to ModuleBase::OnError). Override it to unlock instruments this module depends on (via RunnableInstance::UnlockObject()) and to deregister/unsubscribe from events (via InterModuleEvent::Deregister()).
void	OnTerminate (DynExp::ModuleInstance *Instance, bool) const
void	OnStopAction (DynExp::ModuleInstance *Instance, bool) const
void	OnSetHomePosition (DynExp::ModuleInstance *Instance, bool) const
void	OnGoToHomePosition (DynExp::ModuleInstance *Instance, bool) const
void	OnToggleLEDLightSource (DynExp::ModuleInstance *Instance, bool State) const
void	OnTogglePumpLightSource (DynExp::ModuleInstance *Instance, bool State) const
void	OnSetupModeChanged (DynExp::ModuleInstance *Instance, QAction *Action) const
void	OnAutofocus (DynExp::ModuleInstance *Instance, bool) const
void	OnOptimizePositions (DynExp::ModuleInstance *Instance, bool) const
void	OnToggleHBTMirror (DynExp::ModuleInstance *Instance, bool Checked) const
void	OnResetCellID (DynExp::ModuleInstance *Instance, bool) const
void	OnGeneralWidefieldPowerChanged (DynExp::ModuleInstance *Instance, double Value) const
void	OnGeneralConfocalPowerChanged (DynExp::ModuleInstance *Instance, double Value) const
void	OnGeneralFocusCurrentVoltageChanged (DynExp::ModuleInstance *Instance, double Value) const
void	OnGeneralFocusZeroVoltageChanged (DynExp::ModuleInstance *Instance, double Value) const
void	OnGeneralFocusConfocalOffsetVoltageChanged (DynExp::ModuleInstance *Instance, double Value) const
void	OnGeneralSetZeroFocus (DynExp::ModuleInstance *Instance, bool) const
void	OnGeneralApplyZeroFocus (DynExp::ModuleInstance *Instance, bool) const
void	OnWidefieldLEDExposureTimeChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnWidefieldApplyLEDExposureTime (DynExp::ModuleInstance *Instance, bool) const
void	OnWidefieldPumpExposureTimeChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnWidefieldApplyPumpExposureTime (DynExp::ModuleInstance *Instance, bool) const
void	OnWidefieldFindConfocalSpot (DynExp::ModuleInstance *Instance, bool) const
void	OnCaptureLEDImage (DynExp::ModuleInstance *Instance, bool) const
void	OnCaptureWidefieldImage (DynExp::ModuleInstance *Instance, bool) const
void	OnWidefieldReadCellID (DynExp::ModuleInstance *Instance, bool) const
void	OnWidefieldAnalyzeImageDistortion (DynExp::ModuleInstance *Instance, bool) const
void	OnWidefieldLocalizeEmitters (DynExp::ModuleInstance *Instance, bool) const
void	OnWidefieldImageClicked (DynExp::ModuleInstance *Instance, QPoint Position) const
void	OnConfocalConfocalWidthChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnConfocalConfocalHeightChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnConfocalConfocalDistPerPixelChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnConfocalSPDExposureTimeChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnConfocalOptimizationInitXYStepSizeChanged (DynExp::ModuleInstance *Instance, double Value) const
void	OnConfocalOptimizationInitZStepSizeChanged (DynExp::ModuleInstance *Instance, double Value) const
void	OnConfocalOptimizationToleranceChanged (DynExp::ModuleInstance *Instance, double Value) const
void	OnPerformConfocalScan (DynExp::ModuleInstance *Instance, bool) const
void	ConfocalSurfaceSelectedPointChanged (DynExp::ModuleInstance *Instance, QPoint Position) const
void	OnHBTBinWidthChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnHBTBinCountChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnHHBTMaxIntegrationTimeChanged (DynExp::ModuleInstance *Instance, double Value) const
void	OnMeasureHBT (DynExp::ModuleInstance *Instance, bool) const
void	OnImageCapturingPaused (DynExp::ModuleInstance *Instance) const
void	OnFinishedAutofocus (DynExp::ModuleInstance *Instance, bool Success, double Voltage) const
void	OnSpectrumFinishedRecording (DynExp::ModuleInstance *Instance) const
void	OnPLEAcquisitionFinished (DynExp::ModuleInstance *Instance) const
void	OnAutoMeasureSavePathChanged (DynExp::ModuleInstance *Instance, QString Path) const
void	OnAutoMeasureNumberImageSetsChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnAutoMeasureInitialImageSetWaitTimeChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnAutoMeasureImagePositionScatterRadius (DynExp::ModuleInstance *Instance, int Value) const
void	OnAutoMeasureLocalizationTypeChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnToggleAutoMeasureWidefieldPLEEnabled (DynExp::ModuleInstance *Instance, bool State) const
void	OnToggleAutoMeasureOptimizeEnabled (DynExp::ModuleInstance *Instance, bool State) const
void	OnToggleAutoMeasureSpectrumEnabled (DynExp::ModuleInstance *Instance, bool State) const
void	OnToggleAutoMeasureConfocalPLEEnabled (DynExp::ModuleInstance *Instance, bool State) const
void	OnToggleAutoMeasureHBTEnabled (DynExp::ModuleInstance *Instance, bool State) const
void	OnAutoMeasureNumOptimizationAttemptsChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnAutoMeasureMaxOptimizationRerunsChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnAutoMeasureOptimizationMaxDistanceChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnAutoMeasureCountRateThresholdChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnAutoMeasureCellRangeFromXChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnAutoMeasureCellRangeFromYChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnAutoMeasureCellRangeToXChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnAutoMeasureCellRangeToYChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnAutoMeasureCellSkipXChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnAutoMeasureCellSkipYChanged (DynExp::ModuleInstance *Instance, int Value) const
void	OnToggleAutoMeasureSampleRotated (DynExp::ModuleInstance *Instance, Qt::CheckState State) const
void	OnAutoMeasureRunLocalization (DynExp::ModuleInstance *Instance, bool) const
void	OnAutoMeasureRunCharacterization (DynExp::ModuleInstance *Instance, bool) const
void	OnAutoMeasureRunSampleCharacterization (DynExp::ModuleInstance *Instance, bool) const
StateType	ReturnToReadyStateFunc (DynExp::ModuleInstance &Instance)
StateType	InitializingStateFunc (DynExp::ModuleInstance &Instance)
StateType	SetupTransitionBeginStateFunc (DynExp::ModuleInstance &Instance)
StateType	SetupTransitioningStateFunc (DynExp::ModuleInstance &Instance)
StateType	SetupTransitionEndStateFunc (DynExp::ModuleInstance &Instance)
StateType	ReadyStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutofocusBeginStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutofocusWaitingStateFunc (DynExp::ModuleInstance &Instance)
StateType	LEDImageAcquisitionBeginStateFunc (DynExp::ModuleInstance &Instance)
StateType	WidefieldImageAcquisitionBeginStateFunc (DynExp::ModuleInstance &Instance)
StateType	WaitingForImageReadyToCaptureStateFunc (DynExp::ModuleInstance &Instance)
StateType	WaitingForImageStateFunc (DynExp::ModuleInstance &Instance)
StateType	WaitingForWidefieldCellIDStateFunc (DynExp::ModuleInstance &Instance)
StateType	WidefieldCellWaitUntilCenteredStateFunc (DynExp::ModuleInstance &Instance)
StateType	WaitingForWidefieldLocalizationStateFunc (DynExp::ModuleInstance &Instance)
StateType	FindingConfocalSpotBeginStateFunc (DynExp::ModuleInstance &Instance)
StateType	FindingConfocalSpotAfterTransitioningToConfocalModeStateFunc (DynExp::ModuleInstance &Instance)
StateType	FindingConfocalSpotAfterRecordingWidefieldImageStateFunc (DynExp::ModuleInstance &Instance)
StateType	ConfocalScanStepStateFunc (DynExp::ModuleInstance &Instance)
StateType	ConfocalScanWaitUntilMovedStateFunc (DynExp::ModuleInstance &Instance)
StateType	ConfocalScanCaptureStateFunc (DynExp::ModuleInstance &Instance)
StateType	ConfocalScanWaitUntilCapturedStateFunc (DynExp::ModuleInstance &Instance)
StateType	ConfocalOptimizationInitStateFunc (DynExp::ModuleInstance &Instance)
StateType	ConfocalOptimizationInitSubStepStateFunc (DynExp::ModuleInstance &Instance)
StateType	ConfocalOptimizationWaitStateFunc (DynExp::ModuleInstance &Instance)
StateType	ConfocalOptimizationStepStateFunc (DynExp::ModuleInstance &Instance)
StateType	HBTAcquiringStateFunc (DynExp::ModuleInstance &Instance)
StateType	WaitingStateFunc (DynExp::ModuleInstance &Instance)
StateType	PLEAcquisitionWaitingStateFunc (DynExp::ModuleInstance &Instance)
StateType	SpectrumAcquisitionWaitingStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureLocalizationStepStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureLocalizationSaveLEDImageStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureLocalizationSaveWidefieldImageStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureLocalizationMovingStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureLocalizationPLEBeginStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureLocalizationPLEFinishedStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureCharacterizationStepStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureCharacterizationGotoEmitterStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureCharacterizationOptimizationFinishedStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureCharacterizationSpectrumBeginStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureCharacterizationSpectrumFinishedStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureCharacterizationPLEBeginStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureCharacterizationPLEFinishedStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureCharacterizationHBTBeginStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureCharacterizationHBTWaitForInitStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureCharacterizationHBTFinishedStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureSampleStepStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureSampleReadCellIDStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureSampleReadCellIDFinishedStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureSampleLocalizeStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureSampleFindEmittersStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureSampleCharacterizeStateFunc (DynExp::ModuleInstance &Instance)
StateType	AutoMeasureSampleAdvanceCellStateFunc (DynExp::ModuleInstance &Instance)
	Checks whether the last cell within the sample characterization range (specified as a square region by WidefieldMicroscopeData::AutoMeasureCellRangeFrom and WidefieldMicroscopeData::AutoMeasureCellRangeTo) has been reached. If this is not the case, the sample is moved to advance to the next cell. To advance, the x-coordinate is increased. After reaching the end of the current line (given by WidefieldMicroscopeData::AutoMeasureCellRangeTo::x), a 'line break' is performed by increasing the y-coordinate and resetting the x-coordinate to WidefieldMicroscopeData::AutoMeasureCellRangeFrom::x.

Static Private Member Functions
template<typename PosT >
static ModuleDataType::PositionPoint	PositionPointFromPixelDist (Util::SynchronizedPointer< const ParamsType > &ModuleParams, Util::SynchronizedPointer< ModuleDataType > &ModuleData, const PosT x, const PosT y)
static ConfocalOptimizationThreadReturnType	ConfocalOptimizationThread (WidefieldMicroscope *Owner)
static double	ConfocalOptimizationFuncForwarder (const gsl_vector *vector, void *params)

Private Attributes
const Util::StateMachineContext< StateMachineStateType >	AutofocusSetupTransitioningContext
const Util::StateMachineContext< StateMachineStateType >	FindingConfocalSpotBeginContext
const Util::StateMachineContext< StateMachineStateType >	FindingConfocalSpotRecordingWidefieldImageContext
const Util::StateMachineContext< StateMachineStateType >	ConfocalOptimizationContext
const Util::StateMachineContext< StateMachineStateType >	LEDImageAcquisitionSetupTransitioningContext
const Util::StateMachineContext< StateMachineStateType >	WidefieldImageAcquisitionSetupTransitioningContext
const Util::StateMachineContext< StateMachineStateType >	AutoMeasureLocalizationContext
const Util::StateMachineContext< StateMachineStateType >	AutoMeasureCharacterizationContext
const Util::StateMachineContext< StateMachineStateType >	AutoMeasureCharacterizationOptimizationContext
const Util::StateMachineContext< StateMachineStateType >	AutoMeasureCharacterizationSpectrumContext
const Util::StateMachineContext< StateMachineStateType >	AutoMeasureCharacterizationPLEContext
const Util::StateMachineContext< StateMachineStateType >	AutoMeasureCharacterizationHBTContext
const Util::StateMachineContext< StateMachineStateType >	AutoMeasureSampleContext
const Util::StateMachineContext< StateMachineStateType >	AutoMeasureSampleRecenterCellContext
const Util::StateMachineContext< StateMachineStateType >	AutoMeasureSampleLocalizationContext
const Util::StateMachineContext< StateMachineStateType >	AutoMeasureSampleCharacterizationContext
const Util::StateMachineContext< StateMachineStateType >	AutoMeasureSampleCharacterizationOptimizationContext
const Util::StateMachineContext< StateMachineStateType >	AutoMeasureSampleCharacterizationSpectrumContext
const Util::StateMachineContext< StateMachineStateType >	AutoMeasureSampleCharacterizationPLEContext
const Util::StateMachineContext< StateMachineStateType >	AutoMeasureSampleCharacterizationHBTContext
Util::StateMachine< StateMachineStateType >	StateMachine
const std::shared_ptr< AtomicPositionerStateType >	ConfocalScanPositionerStateX
const std::shared_ptr< AtomicPositionerStateType >	ConfocalScanPositionerStateY
const std::shared_ptr< AtomicPositionerStateType >	ConfocalScanPositionerStateZ
const std::shared_ptr< AtomicWidefieldImageProcessingStateType >	WidefieldCellIDState
const std::shared_ptr< AtomicWidefieldImageProcessingStateType >	WidefieldLocalizationState
std::list< WidefieldMicroscopeData::PositionPoint >	ConfocalScanPositions
const gsl_multimin_fminimizer_type *const	GSLConfocalOptimizationMinimizer = gsl_multimin_fminimizer_nmsimplex2
gsl_multimin_function	GSLConfocalOptimizationFuncDesc { &WidefieldMicroscope::ConfocalOptimizationFuncForwarder, GSLConfocalOptimizationNumDimensions, this }
gsl_multimin_fminimizer *const	GSLConfocalOptimizationState
gsl_vector *const	GSLConfocalOptimizationStepSize
gsl_vector *const	GSLConfocalOptimizationInitialPoint
std::atomic< size_t >	ConfocalOptimizationNumStepsPerformed = 0
std::atomic_bool	ConfocalOptimizationPromisesRenewed = false
std::promise< ConfocalOptimizationStateType >	ConfocalOptimizationStatePromise
std::future< ConfocalOptimizationStateType >	ConfocalOptimizationStateFuture
std::promise< ConfocalOptimizationFeedbackType >	ConfocalOptimizationFeedbackPromise
std::future< ConfocalOptimizationFeedbackType >	ConfocalOptimizationFeedbackFuture
std::future< ConfocalOptimizationThreadReturnType >	ConfocalOptimizationThreadReturnFuture
std::chrono::system_clock::time_point	SetupTransitionFinishedTimePoint
bool	TurnOnPumpSourceAfterTransitioning = false
bool	ImageCapturingPaused = false
std::chrono::system_clock::time_point	WaitingEndTimePoint
std::chrono::microseconds	HBTIntegrationTimeBeforeReset { 0 }
size_t	NumFailedUpdateAttempts = 0
bool	LogUIMessagesOnly = false

Static Private Attributes
static constexpr auto	InitializingState
static constexpr auto	SetupTransitionBeginState
static constexpr auto	SetupTransitioningState
static constexpr auto	SetupTransitionEndState
static constexpr auto	SetupTransitionFinishedState
static constexpr auto	ReadyState
static constexpr auto	AutofocusBeginState
static constexpr auto	AutofocusWaitingState
static constexpr auto	AutofocusFinishedState
static constexpr auto	LEDImageAcquisitionBeginState
static constexpr auto	WidefieldImageAcquisitionBeginState
static constexpr auto	WaitingForLEDImageReadyToCaptureState
static constexpr auto	WaitingForLEDImageState
static constexpr auto	WaitingForLEDImageFinishedState
static constexpr auto	WaitingForWidefieldImageReadyToCaptureState
static constexpr auto	WaitingForWidefieldImageState
static constexpr auto	WaitingForWidefieldImageFinishedState
static constexpr auto	WaitingForWidefieldCellIDState
static constexpr auto	WidefieldCellWaitUntilCenteredState
static constexpr auto	WidefieldCellIDReadFinishedState
static constexpr auto	WaitingForWidefieldLocalizationState
static constexpr auto	WidefieldLocalizationFinishedState
static constexpr auto	FindingConfocalSpotBeginState
static constexpr auto	FindingConfocalSpotAfterTransitioningToConfocalModeState
static constexpr auto	FindingConfocalSpotAfterRecordingWidefieldImageState
static constexpr auto	ConfocalScanStepState
static constexpr auto	ConfocalScanWaitUntilMovedState
static constexpr auto	ConfocalScanCaptureState
static constexpr auto	ConfocalScanWaitUntilCapturedState
static constexpr auto	ConfocalOptimizationInitState
static constexpr auto	ConfocalOptimizationInitSubStepState
static constexpr auto	ConfocalOptimizationWaitState
static constexpr auto	ConfocalOptimizationStepState
static constexpr auto	ConfocalOptimizationFinishedState
static constexpr auto	HBTAcquiringState
static constexpr auto	HBTFinishedState
static constexpr auto	WaitingState
static constexpr auto	WaitingFinishedState
static constexpr auto	PLEAcquisitionWaitingState
static constexpr auto	PLEAcquisitionFinishedState
static constexpr auto	SpectrumAcquisitionWaitingState
static constexpr auto	SpectrumAcquisitionFinishedState
static constexpr auto	AutoMeasureLocalizationStepState
static constexpr auto	AutoMeasureLocalizationSaveLEDImageState
static constexpr auto	AutoMeasureLocalizationSaveWidefieldImageState
static constexpr auto	AutoMeasureLocalizationMovingState
static constexpr auto	AutoMeasureLocalizationPLEBeginState
static constexpr auto	AutoMeasureLocalizationPLEFinishedState
static constexpr auto	AutoMeasureLocalizationFinishedState
static constexpr auto	AutoMeasureCharacterizationStepState
static constexpr auto	AutoMeasureCharacterizationGotoEmitterState
static constexpr auto	AutoMeasureCharacterizationOptimizationFinishedState
static constexpr auto	AutoMeasureCharacterizationSpectrumBeginState
static constexpr auto	AutoMeasureCharacterizationSpectrumFinishedState
static constexpr auto	AutoMeasureCharacterizationPLEBeginState
static constexpr auto	AutoMeasureCharacterizationPLEFinishedState
static constexpr auto	AutoMeasureCharacterizationHBTBeginState
static constexpr auto	AutoMeasureCharacterizationHBTWaitForInitState
static constexpr auto	AutoMeasureCharacterizationHBTFinishedState
static constexpr auto	AutoMeasureCharacterizationFinishedState
static constexpr auto	AutoMeasureSampleStepState
static constexpr auto	AutoMeasureSampleReadCellIDState
static constexpr auto	AutoMeasureSampleReadCellIDFinishedState
static constexpr auto	AutoMeasureSampleLocalizeState
static constexpr auto	AutoMeasureSampleFindEmittersState
static constexpr auto	AutoMeasureSampleCharacterizeState
static constexpr auto	AutoMeasureSampleAdvanceCellState
static constexpr auto	AutoMeasureSampleFinishedState
static constexpr size_t	GSLConfocalOptimizationNumDimensions = 3

Additional Inherited Members
Public Attributes inherited from DynExp::ModuleBase
ModuleThreadOnlyType	ModuleThreadOnly
	Allow exclusive access to some of ModuleBase's private methods to the module thread ModuleThreadMain().
EventListenersOnlyType	EventListenersOnly
	Allow exclusive access to some of ModuleBase's private methods to any TypedEventListeners class.
Public Attributes inherited from DynExp::RunnableObject
RunnableInstanceOnlyType	RunnableInstanceOnly
	Allow exclusive access to some of RunnableObject's private methods to class RunnableInstance.
Public Attributes inherited from DynExp::Object
LinkedObjectWrapperOnlyType	LinkedObjectWrapperOnly
	Allow exclusive access to some of Object's private methods to any LinkedObjectWrapper<T>.
Static Public Attributes inherited from DynExp::ModuleBase
static constexpr auto	GetModuleDataTimeoutDefault = std::chrono::milliseconds(1000)
	Determines the default timeout for GetModuleData() to lock the mutex synchronizing the module's data ModuleData.
Static Public Attributes inherited from DynExp::RunnableObject
static constexpr auto	ShortTimeoutDefault = std::chrono::milliseconds(50)
	Default timeout e.g. used as a default for calls to InstrumentBase::GetInstrumentData or ModuleBase::GetModuleData.
static constexpr auto	TerminateTimeoutDefault = std::chrono::milliseconds(3000)
	Default timeout used as a default for calls to RunnableObject::Terminate.
Static Public Attributes inherited from DynExp::Object
static constexpr std::chrono::milliseconds	GetParamsTimeoutDefault = std::chrono::milliseconds(100)
	Default timeout used by Object::GetParams() to lock the mutex of the parameter instance assigned to this Object instance.
Protected Types inherited from DynExp::RunnableObject
using	ThreadFuncType = std::function< int(std::unique_ptr< RunnableInstance > &&, RunnableObject *)>
	Type of the thread function executed by RunnableObject instances.
Protected Member Functions inherited from DynExp::QModuleBase
template<typename SenderType , typename SignalType , typename ReceiverType , typename EventType >
void	Connect (SenderType *Sender, SignalType Signal, ReceiverType *Receiver, EventType Event)
	Uses Qt's connect mechanism to connect a QObject's signal to a DynExp module's event. By directly connecting slots to signals via QObject::connect(), the slot function runs in the user interface thread. In contrast, this function connects a signal to a lambda function, which itself enqueues an event in the module's event queue. The event runs in the module thread. Arguments passed from the signal to the lambda function are forwarded to the event.
template<typename WidgetType = QModuleWidget>
WidgetType *	GetWidget () const
	Getter for Widget.
Protected Member Functions inherited from DynExp::RunnableObject
void	Init ()
	Initializes member variables in case of a reset.
std::promise< void >	MakeThreadExitedPromise ()
	Helper function to be used by overridden RunChild() functions in derived classes to (re)initialize the ThreadExitedSignal future and to provide the derived class a promise to signal the RunnableObject instance that its thread has terminated.
void	MakeThread (ThreadFuncType ThreadFunc, std::unique_ptr< RunnableInstance > &&InstancePtr)
	Creates and runs the thread of the RunnableObject instance. Call this function in the derived class overriding RunChild() and nowhere else!
bool	IsCallFromRunnableThread () const
	Checks whether Thread's id matches the id of the calling thread. This is thread-safe if the function is called by the RunnableObject instance's thread since Terminate() joins the threads before changing the Thread member. It is also thread-safe if the function is called by the thread owning the the RunnableObject instance since Run() and Terminate() can only be called by this thread. Only Run() and Terminate() (indirectly) modify Thread.
void	EnsureCallFromRunnableThread () const
	Asserts that the call to this function is performed from the RunnableObject instance's thread by calling IsCallFromRunnableThread().
void	SetReasonWhyPaused (std::string Description)
	Sets the reason why this RunnableObject instance has been paused.
void	SetReasonWhyPaused (const Util::Exception &e)
	Sets the reason why this RunnableObject instance has been paused.
void	ClearReasonWhyPaused ()
	Removes the reason why this RunnableObject instance has been paused (since it is resumed).
Protected Member Functions inherited from Util::INonCopyable
constexpr	INonCopyable ()=default
	~INonCopyable ()=default
Protected Member Functions inherited from DynExp::Object
	Object (const std::thread::id OwnerThreadID, ParamsBasePtrType &&Params)
	Constructs an Object instance.
virtual	~Object ()=0
void	EnsureCallFromOwningThread () const
	Asserts that the call to this function is performed from the thread which constructed this Object instance (the thread with the id stored in Object::OwnerThreadID).
ParamsTypeSyncPtrType	GetNonConstParams (const std::chrono::milliseconds Timeout=GetParamsTimeoutDefault) const
	Allows derived Objects to edit their own parameters - even in const task functions (for instruments) or event functions (for modules).
auto	LockUserList (const std::chrono::milliseconds Timeout=Util::ILockable::DefaultTimeout)
	Locks the user list for thread-safe manipulation.
void	DeregisterAllUnsafe ()
	Deregisters all users and notifies them that they need to check the states of their used linked objects.
auto	GetUseCountUnsafe ()
auto	GetUserNamesStringUnsafe () const
bool	IsUnusedUnsafe ()
	Returns whether this Object instance is used by other instances (not thread-safe).
Static Protected Member Functions inherited from DynExp::ModuleBase
static auto	GetExceptionUnsafe (const ModuleDataTypeSyncPtrConstType &ModuleDataPtr)
	Getter for ModuleDataBase::ModuleException assuming that the module data has already been locked.

Detailed Description

Definition at line 560 of file WidefieldMicroscope.h.

---

Class Documentation

DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationStateType

struct DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationStateType

Definition at line 645 of file WidefieldMicroscope.h.

Class Members
PositionType	X {}
PositionType	Y {}
SampleType	Z {}

Member Typedef Documentation

AtomicPositionerStateType

using DynExpModule::Widefield::WidefieldMicroscope::AtomicPositionerStateType = std::atomic<PositionerStateType>

private

Definition at line 565 of file WidefieldMicroscope.h.

AtomicWidefieldImageProcessingStateType

using DynExpModule::Widefield::WidefieldMicroscope::AtomicWidefieldImageProcessingStateType = std::atomic<WidefieldImageProcessingStateType>

private

Definition at line 566 of file WidefieldMicroscope.h.

ConfigType

using DynExpModule::Widefield::WidefieldMicroscope::ConfigType = WidefieldMicroscopeConfigurator

Definition at line 570 of file WidefieldMicroscope.h.

ConfocalOptimizationFeedbackType

using DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationFeedbackType = double

private

Definition at line 654 of file WidefieldMicroscope.h.

ModuleDataType

using DynExpModule::Widefield::WidefieldMicroscope::ModuleDataType = WidefieldMicroscopeData

Definition at line 571 of file WidefieldMicroscope.h.

ParamsType

using DynExpModule::Widefield::WidefieldMicroscope::ParamsType = WidefieldMicroscopeParams

Definition at line 569 of file WidefieldMicroscope.h.

Member Enumeration Documentation

ConfocalOptimizationThreadReturnType

enum class DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationThreadReturnType

strongprivate

Enumerator
NextStep
Finished
Failed

Definition at line 652 of file WidefieldMicroscope.h.

PositionerStateType

enum class DynExpModule::Widefield::WidefieldMicroscope::PositionerStateType

strongprivate

Enumerator
WaitingForMovement
Moving
Arrived

Definition at line 562 of file WidefieldMicroscope.h.

WidefieldImageProcessingStateType

enum class DynExpModule::Widefield::WidefieldMicroscope::WidefieldImageProcessingStateType

strongprivate

Enumerator
Waiting
Finished
Failed

Definition at line 563 of file WidefieldMicroscope.h.

Constructor & Destructor Documentation

WidefieldMicroscope()

DynExpModule::Widefield::WidefieldMicroscope::WidefieldMicroscope	(	const std::thread::id	OwnerThreadID,
		DynExp::ParamsBasePtrType &&	Params
	)

Definition at line 540 of file WidefieldMicroscope.cpp.

~WidefieldMicroscope()

DynExpModule::Widefield::WidefieldMicroscope::~WidefieldMicroscope ( )

virtual

Definition at line 583 of file WidefieldMicroscope.cpp.

Member Function Documentation

AutofocusBeginStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutofocusBeginStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2252 of file WidefieldMicroscope.cpp.

AutofocusWaitingStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutofocusWaitingStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2275 of file WidefieldMicroscope.cpp.

AutoMeasureCharacterizationGotoEmitterStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationGotoEmitterStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2893 of file WidefieldMicroscope.cpp.

AutoMeasureCharacterizationHBTBeginStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationHBTBeginStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 3049 of file WidefieldMicroscope.cpp.

AutoMeasureCharacterizationHBTFinishedStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationHBTFinishedStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 3076 of file WidefieldMicroscope.cpp.

AutoMeasureCharacterizationHBTWaitForInitStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationHBTWaitForInitStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 3058 of file WidefieldMicroscope.cpp.

AutoMeasureCharacterizationOptimizationFinishedStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationOptimizationFinishedStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2923 of file WidefieldMicroscope.cpp.

AutoMeasureCharacterizationPLEBeginStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationPLEBeginStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 3015 of file WidefieldMicroscope.cpp.

AutoMeasureCharacterizationPLEFinishedStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationPLEFinishedStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 3026 of file WidefieldMicroscope.cpp.

AutoMeasureCharacterizationSpectrumBeginStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationSpectrumBeginStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2981 of file WidefieldMicroscope.cpp.

AutoMeasureCharacterizationSpectrumFinishedStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationSpectrumFinishedStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2992 of file WidefieldMicroscope.cpp.

AutoMeasureCharacterizationStepStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationStepStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2850 of file WidefieldMicroscope.cpp.

AutoMeasureLocalizationMovingStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureLocalizationMovingStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2825 of file WidefieldMicroscope.cpp.

AutoMeasureLocalizationPLEBeginStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureLocalizationPLEBeginStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2835 of file WidefieldMicroscope.cpp.

AutoMeasureLocalizationPLEFinishedStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureLocalizationPLEFinishedStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2845 of file WidefieldMicroscope.cpp.

AutoMeasureLocalizationSaveLEDImageStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureLocalizationSaveLEDImageStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2773 of file WidefieldMicroscope.cpp.

AutoMeasureLocalizationSaveWidefieldImageStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureLocalizationSaveWidefieldImageStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2790 of file WidefieldMicroscope.cpp.

AutoMeasureLocalizationStepStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureLocalizationStepStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2758 of file WidefieldMicroscope.cpp.

AutoMeasureSampleAdvanceCellStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleAdvanceCellStateFunc ( DynExp::ModuleInstance &  Instance )

private

Checks whether the last cell within the sample characterization range (specified as a square region by WidefieldMicroscopeData::AutoMeasureCellRangeFrom and WidefieldMicroscopeData::AutoMeasureCellRangeTo) has been reached. If this is not the case, the sample is moved to advance to the next cell. To advance, the x-coordinate is increased. After reaching the end of the current line (given by WidefieldMicroscopeData::AutoMeasureCellRangeTo::x), a 'line break' is performed by increasing the y-coordinate and resetting the x-coordinate to WidefieldMicroscopeData::AutoMeasureCellRangeFrom::x.

Parameters

Instance

Handle to current WidefieldMicroscope instance

Returns

StateType::AutoMeasureSampleFinished when finished, StateType::AutoMeasureSampleStep otherwise

Definition at line 3208 of file WidefieldMicroscope.cpp.

AutoMeasureSampleCharacterizeStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleCharacterizeStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 3191 of file WidefieldMicroscope.cpp.

AutoMeasureSampleFindEmittersStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleFindEmittersStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 3178 of file WidefieldMicroscope.cpp.

AutoMeasureSampleLocalizeStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleLocalizeStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 3160 of file WidefieldMicroscope.cpp.

AutoMeasureSampleReadCellIDFinishedStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleReadCellIDFinishedStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 3117 of file WidefieldMicroscope.cpp.

AutoMeasureSampleReadCellIDStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleReadCellIDStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 3110 of file WidefieldMicroscope.cpp.

AutoMeasureSampleStepStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleStepStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 3099 of file WidefieldMicroscope.cpp.

BringMarkerToConfocalSpot()

void DynExpModule::Widefield::WidefieldMicroscope::BringMarkerToConfocalSpot ( Util::SynchronizedPointer< const ParamsType > &  ModuleParams, Util::SynchronizedPointer< ModuleDataType > &  ModuleData, QPoint  MarkerPos, QPointF  SamplePos  ) const

private

Definition at line 933 of file WidefieldMicroscope.cpp.

BuildFilename()

std::filesystem::path DynExpModule::Widefield::WidefieldMicroscope::BuildFilename ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData, std::string_view  FilenameSuffix  ) const

private

Definition at line 1211 of file WidefieldMicroscope.cpp.

CalcMarkerToConfocalSpotDestiny()

WidefieldMicroscopeData::PositionPoint DynExpModule::Widefield::WidefieldMicroscope::CalcMarkerToConfocalSpotDestiny ( Util::SynchronizedPointer< const ParamsType > &  ModuleParams, Util::SynchronizedPointer< ModuleDataType > &  ModuleData, QPoint  MarkerPos, QPointF  SamplePos  ) const

private

Definition at line 924 of file WidefieldMicroscope.cpp.

CalculateConfocalScanPositions()

QSurfaceDataArray DynExpModule::Widefield::WidefieldMicroscope::CalculateConfocalScanPositions ( const int  Width, const int  Height, const int  DistPerPixel, const WidefieldMicroscopeData::PositionPoint  CenterPosition  ) const

private

Definition at line 939 of file WidefieldMicroscope.cpp.

Category()

static constexpr auto DynExpModule::Widefield::WidefieldMicroscope::Category ( )

inlinestaticconstexprnoexcept

Definition at line 574 of file WidefieldMicroscope.h.

ConfocalOptimizationFunc()

double DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationFunc ( const gsl_vector *  vector )

privatenoexcept

Definition at line 1288 of file WidefieldMicroscope.cpp.

ConfocalOptimizationFuncForwarder()

double DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationFuncForwarder ( const gsl_vector *  vector, void *  params  )

staticprivate

Definition at line 1283 of file WidefieldMicroscope.cpp.

ConfocalOptimizationInitPromises()

void DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationInitPromises ( )

private

Definition at line 1235 of file WidefieldMicroscope.cpp.

ConfocalOptimizationInitStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationInitStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2615 of file WidefieldMicroscope.cpp.

ConfocalOptimizationInitSubStepStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationInitSubStepStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2624 of file WidefieldMicroscope.cpp.

ConfocalOptimizationStepStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationStepStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2655 of file WidefieldMicroscope.cpp.

ConfocalOptimizationThread()

WidefieldMicroscope::ConfocalOptimizationThreadReturnType DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationThread ( WidefieldMicroscope *  Owner )

staticprivate

Definition at line 1247 of file WidefieldMicroscope.cpp.

ConfocalOptimizationWaitStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationWaitStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2631 of file WidefieldMicroscope.cpp.

ConfocalScanCaptureStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::ConfocalScanCaptureStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2542 of file WidefieldMicroscope.cpp.

ConfocalScanStepStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::ConfocalScanStepStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2512 of file WidefieldMicroscope.cpp.

ConfocalScanWaitUntilCapturedStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::ConfocalScanWaitUntilCapturedStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2568 of file WidefieldMicroscope.cpp.

ConfocalScanWaitUntilMovedStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::ConfocalScanWaitUntilMovedStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2529 of file WidefieldMicroscope.cpp.

ConfocalSurfaceSelectedPointChanged()

void DynExpModule::Widefield::WidefieldMicroscope::ConfocalSurfaceSelectedPointChanged ( DynExp::ModuleInstance *  Instance, QPoint  Position  ) const

private

Definition at line 1905 of file WidefieldMicroscope.cpp.

FindingConfocalSpotAfterRecordingWidefieldImageStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::FindingConfocalSpotAfterRecordingWidefieldImageStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2486 of file WidefieldMicroscope.cpp.

FindingConfocalSpotAfterTransitioningToConfocalModeStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::FindingConfocalSpotAfterTransitioningToConfocalModeStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2471 of file WidefieldMicroscope.cpp.

FindingConfocalSpotBeginStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::FindingConfocalSpotBeginStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2454 of file WidefieldMicroscope.cpp.

GetCategory()

virtual std::string DynExpModule::Widefield::WidefieldMicroscope::GetCategory ( ) const

inlineoverridevirtual

Returns the category of this Object type.

Reimplemented from DynExp::ModuleBase.

Definition at line 580 of file WidefieldMicroscope.h.

GetMainLoopDelay()

std::chrono::milliseconds DynExpModule::Widefield::WidefieldMicroscope::GetMainLoopDelay ( ) const

finaloverridevirtual

Specifies in which time intervals the module's event queue runs to handle pending events.

Returns

Delay time in between event queue executions. Return std::chrono::milliseconds::max() to make the module thread only wake up to execute the module main loop (after handling enqueued events) when an event is enqueued. Return 0 to make the module main loop execute as fast as possible.

Reimplemented from DynExp::ModuleBase.

Definition at line 593 of file WidefieldMicroscope.cpp.

GetName()

virtual std::string DynExpModule::Widefield::WidefieldMicroscope::GetName ( ) const

inlineoverridevirtual

Returns the name of this Object type.

Implements DynExp::Object.

Definition at line 579 of file WidefieldMicroscope.h.

HBTAcquiringStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::HBTAcquiringStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2701 of file WidefieldMicroscope.cpp.

InitializeConfocalOptimizer()

void DynExpModule::Widefield::WidefieldMicroscope::InitializeConfocalOptimizer ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData ) const

private

Definition at line 1086 of file WidefieldMicroscope.cpp.

InitializeHBT()

void DynExpModule::Widefield::WidefieldMicroscope::InitializeHBT ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData ) const

private

Definition at line 1111 of file WidefieldMicroscope.cpp.

InitializingStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::InitializingStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2175 of file WidefieldMicroscope.cpp.

InitiateLocalizationFromImage()

StateType DynExpModule::Widefield::WidefieldMicroscope::InitiateLocalizationFromImage ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData ) const

private

Definition at line 1035 of file WidefieldMicroscope.cpp.

InitiateReadCellIDFromImage()

StateType DynExpModule::Widefield::WidefieldMicroscope::InitiateReadCellIDFromImage ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData ) const

private

Definition at line 1003 of file WidefieldMicroscope.cpp.

IsCharacterizingSample()

bool DynExpModule::Widefield::WidefieldMicroscope::IsCharacterizingSample ( ) const

privatenoexcept

Returns true if an entire sample (multiple cells) are processed, false if only a single cell is processed.

Returns whether the microscope is characterizing an entire sample moving from cell to cell. This function is thread-safe since StateMachine::GetContext() reads the current context atomically.

Returns

True if currently characterizing an entire sample, false otherwise.

Definition at line 839 of file WidefieldMicroscope.cpp.

IsReadyState()

bool DynExpModule::Widefield::WidefieldMicroscope::IsReadyState ( ) const

inlineprivatenoexcept

Definition at line 612 of file WidefieldMicroscope.h.

IsSampleMoving()

bool DynExpModule::Widefield::WidefieldMicroscope::IsSampleMoving ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData ) const

private

Checks whether the sample is moving in x- or y-direction. The z-direction is ignored currently since this module doesn't currently make use of it.

Parameters

ModuleData

WidefieldMicroscope's locked module data

Returns

true if the sample is moving, false otherwise

Definition at line 893 of file WidefieldMicroscope.cpp.

LEDImageAcquisitionBeginStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::LEDImageAcquisitionBeginStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2287 of file WidefieldMicroscope.cpp.

MakeUIWidget()

std::unique_ptr< DynExp::QModuleWidget > DynExpModule::Widefield::WidefieldMicroscope::MakeUIWidget ( )

finaloverrideprivatevirtual

Used by InitUI() as a factory function for the module's user interface widget. Create the widget here using std::make_unique and then use Connect() to connect Qt signals to the module's event functions.

Returns

Return the created user interface widget.

Implements DynExp::QModuleBase.

Definition at line 709 of file WidefieldMicroscope.cpp.

ModuleMainLoop()

Util::DynExpErrorCodes::DynExpErrorCodes DynExpModule::Widefield::WidefieldMicroscope::ModuleMainLoop ( DynExp::ModuleInstance &  Instance )

finaloverrideprivatevirtual

Module main loop. The function is executed periodically by the module thread. Also refer to GetMainLoopDelay().

Parameters

Instance

Handle to the module thread's data

Returns

Return Util::DynExpErrorCodes::NoError if module loop should continue, annother error code otherwise.

Implements DynExp::ModuleBase.

Definition at line 662 of file WidefieldMicroscope.cpp.

MoveSampleTo()

void DynExpModule::Widefield::WidefieldMicroscope::MoveSampleTo ( const ModuleDataType::PositionPoint &  Point, Util::SynchronizedPointer< ModuleDataType > &  ModuleData  ) const

private

Definition at line 854 of file WidefieldMicroscope.cpp.

Name()

static constexpr auto DynExpModule::Widefield::WidefieldMicroscope::Name ( )

inlinestaticconstexprnoexcept

Definition at line 573 of file WidefieldMicroscope.h.

OnAutofocus()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutofocus ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1607 of file WidefieldMicroscope.cpp.

OnAutoMeasureCellRangeFromXChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureCellRangeFromXChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 2082 of file WidefieldMicroscope.cpp.

OnAutoMeasureCellRangeFromYChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureCellRangeFromYChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 2088 of file WidefieldMicroscope.cpp.

OnAutoMeasureCellRangeToXChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureCellRangeToXChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 2094 of file WidefieldMicroscope.cpp.

OnAutoMeasureCellRangeToYChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureCellRangeToYChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 2100 of file WidefieldMicroscope.cpp.

OnAutoMeasureCellSkipXChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureCellSkipXChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 2106 of file WidefieldMicroscope.cpp.

OnAutoMeasureCellSkipYChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureCellSkipYChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 2112 of file WidefieldMicroscope.cpp.

OnAutoMeasureCountRateThresholdChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureCountRateThresholdChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 2076 of file WidefieldMicroscope.cpp.

OnAutoMeasureImagePositionScatterRadius()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureImagePositionScatterRadius ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 2016 of file WidefieldMicroscope.cpp.

OnAutoMeasureInitialImageSetWaitTimeChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureInitialImageSetWaitTimeChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 2010 of file WidefieldMicroscope.cpp.

OnAutoMeasureLocalizationTypeChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureLocalizationTypeChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 2022 of file WidefieldMicroscope.cpp.

OnAutoMeasureMaxOptimizationRerunsChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureMaxOptimizationRerunsChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 2064 of file WidefieldMicroscope.cpp.

OnAutoMeasureNumberImageSetsChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureNumberImageSetsChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 2004 of file WidefieldMicroscope.cpp.

OnAutoMeasureNumOptimizationAttemptsChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureNumOptimizationAttemptsChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 2058 of file WidefieldMicroscope.cpp.

OnAutoMeasureOptimizationMaxDistanceChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureOptimizationMaxDistanceChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 2070 of file WidefieldMicroscope.cpp.

OnAutoMeasureRunCharacterization()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureRunCharacterization ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 2134 of file WidefieldMicroscope.cpp.

OnAutoMeasureRunLocalization()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureRunLocalization ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 2124 of file WidefieldMicroscope.cpp.

OnAutoMeasureRunSampleCharacterization()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureRunSampleCharacterization ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 2144 of file WidefieldMicroscope.cpp.

OnAutoMeasureSavePathChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnAutoMeasureSavePathChanged ( DynExp::ModuleInstance *  Instance, QString  Path  ) const

private

Definition at line 1998 of file WidefieldMicroscope.cpp.

OnBringMarkerToConfocalSpot()

void DynExpModule::Widefield::WidefieldMicroscope::OnBringMarkerToConfocalSpot	(	DynExp::ModuleInstance *	Instance,
		QPoint	MarkerPos,
		QPointF	SamplePos
	)		const

Definition at line 644 of file WidefieldMicroscope.cpp.

OnCaptureLEDImage()

void DynExpModule::Widefield::WidefieldMicroscope::OnCaptureLEDImage ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1752 of file WidefieldMicroscope.cpp.

OnCaptureWidefieldImage()

void DynExpModule::Widefield::WidefieldMicroscope::OnCaptureWidefieldImage ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1770 of file WidefieldMicroscope.cpp.

OnConfocalConfocalDistPerPixelChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnConfocalConfocalDistPerPixelChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 1841 of file WidefieldMicroscope.cpp.

OnConfocalConfocalHeightChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnConfocalConfocalHeightChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 1835 of file WidefieldMicroscope.cpp.

OnConfocalConfocalWidthChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnConfocalConfocalWidthChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 1829 of file WidefieldMicroscope.cpp.

OnConfocalOptimizationInitXYStepSizeChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnConfocalOptimizationInitXYStepSizeChanged ( DynExp::ModuleInstance *  Instance, double  Value  ) const

private

Definition at line 1853 of file WidefieldMicroscope.cpp.

OnConfocalOptimizationInitZStepSizeChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnConfocalOptimizationInitZStepSizeChanged ( DynExp::ModuleInstance *  Instance, double  Value  ) const

private

Definition at line 1859 of file WidefieldMicroscope.cpp.

OnConfocalOptimizationToleranceChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnConfocalOptimizationToleranceChanged ( DynExp::ModuleInstance *  Instance, double  Value  ) const

private

Definition at line 1865 of file WidefieldMicroscope.cpp.

OnConfocalSPDExposureTimeChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnConfocalSPDExposureTimeChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 1847 of file WidefieldMicroscope.cpp.

OnExit()

void DynExpModule::Widefield::WidefieldMicroscope::OnExit ( DynExp::ModuleInstance *  Instance ) const

finaloverrideprivatevirtual

This event is triggered right before the module thread terminates (not due to an exception, in this case refer to ModuleBase::OnError). Override it to unlock instruments this module depends on (via RunnableInstance::UnlockObject()) and to deregister/unsubscribe from events (via InterModuleEvent::Deregister()).

Parameters

Instance

Handle to the module thread's data

Reimplemented from DynExp::ModuleBase.

Definition at line 1464 of file WidefieldMicroscope.cpp.

OnFinishedAutofocus()

void DynExpModule::Widefield::WidefieldMicroscope::OnFinishedAutofocus ( DynExp::ModuleInstance *  Instance, bool  Success, double  Voltage  ) const

private

Definition at line 1956 of file WidefieldMicroscope.cpp.

OnGeneralApplyZeroFocus()

void DynExpModule::Widefield::WidefieldMicroscope::OnGeneralApplyZeroFocus ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1698 of file WidefieldMicroscope.cpp.

OnGeneralConfocalPowerChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnGeneralConfocalPowerChanged ( DynExp::ModuleInstance *  Instance, double  Value  ) const

private

Definition at line 1660 of file WidefieldMicroscope.cpp.

OnGeneralFocusConfocalOffsetVoltageChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnGeneralFocusConfocalOffsetVoltageChanged ( DynExp::ModuleInstance *  Instance, double  Value  ) const

private

Definition at line 1683 of file WidefieldMicroscope.cpp.

OnGeneralFocusCurrentVoltageChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnGeneralFocusCurrentVoltageChanged ( DynExp::ModuleInstance *  Instance, double  Value  ) const

private

Definition at line 1668 of file WidefieldMicroscope.cpp.

OnGeneralFocusZeroVoltageChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnGeneralFocusZeroVoltageChanged ( DynExp::ModuleInstance *  Instance, double  Value  ) const

private

Definition at line 1677 of file WidefieldMicroscope.cpp.

OnGeneralSetZeroFocus()

void DynExpModule::Widefield::WidefieldMicroscope::OnGeneralSetZeroFocus ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1692 of file WidefieldMicroscope.cpp.

OnGeneralWidefieldPowerChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnGeneralWidefieldPowerChanged ( DynExp::ModuleInstance *  Instance, double  Value  ) const

private

Definition at line 1652 of file WidefieldMicroscope.cpp.

OnGoToHomePosition()

void DynExpModule::Widefield::WidefieldMicroscope::OnGoToHomePosition ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1560 of file WidefieldMicroscope.cpp.

OnGoToSamplePos()

void DynExpModule::Widefield::WidefieldMicroscope::OnGoToSamplePos	(	DynExp::ModuleInstance *	Instance,
		QPointF	SamplePos
	)		const

Definition at line 631 of file WidefieldMicroscope.cpp.

OnHBTBinCountChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnHBTBinCountChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 1927 of file WidefieldMicroscope.cpp.

OnHBTBinWidthChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnHBTBinWidthChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 1921 of file WidefieldMicroscope.cpp.

OnHHBTMaxIntegrationTimeChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnHHBTMaxIntegrationTimeChanged ( DynExp::ModuleInstance *  Instance, double  Value  ) const

private

Definition at line 1933 of file WidefieldMicroscope.cpp.

OnImageCapturingPaused()

void DynExpModule::Widefield::WidefieldMicroscope::OnImageCapturingPaused ( DynExp::ModuleInstance *  Instance ) const

private

Definition at line 1951 of file WidefieldMicroscope.cpp.

OnInit()

void DynExpModule::Widefield::WidefieldMicroscope::OnInit ( DynExp::ModuleInstance *  Instance ) const

finaloverrideprivatevirtual

This event is triggered right before the module thread starts. Override it to lock instruments this module depends on (via RunnableInstance::LockObject()) and to register/subscribe for events (via InterModuleEvent::Register()).

Parameters

Instance

Handle to the module thread's data

Reimplemented from DynExp::ModuleBase.

Definition at line 1316 of file WidefieldMicroscope.cpp.

OnMeasureHBT()

void DynExpModule::Widefield::WidefieldMicroscope::OnMeasureHBT ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1939 of file WidefieldMicroscope.cpp.

OnOptimizePositions()

void DynExpModule::Widefield::WidefieldMicroscope::OnOptimizePositions ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1617 of file WidefieldMicroscope.cpp.

OnPerformConfocalScan()

void DynExpModule::Widefield::WidefieldMicroscope::OnPerformConfocalScan ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1871 of file WidefieldMicroscope.cpp.

OnPLEAcquisitionFinished()

void DynExpModule::Widefield::WidefieldMicroscope::OnPLEAcquisitionFinished ( DynExp::ModuleInstance *  Instance ) const

private

Definition at line 1993 of file WidefieldMicroscope.cpp.

OnResetCellID()

void DynExpModule::Widefield::WidefieldMicroscope::OnResetCellID ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1643 of file WidefieldMicroscope.cpp.

OnRunCharacterizationFromID()

void DynExpModule::Widefield::WidefieldMicroscope::OnRunCharacterizationFromID	(	DynExp::ModuleInstance *	Instance,
		Util::MarkerGraphicsView::MarkerType::IDType	ID
	)		const

Definition at line 655 of file WidefieldMicroscope.cpp.

OnSaveCurrentImage()

void DynExpModule::Widefield::WidefieldMicroscope::OnSaveCurrentImage	(	DynExp::ModuleInstance *	Instance,
		QString	Filename
	)		const

Definition at line 609 of file WidefieldMicroscope.cpp.

OnSetHomePosition()

void DynExpModule::Widefield::WidefieldMicroscope::OnSetHomePosition ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1550 of file WidefieldMicroscope.cpp.

OnSetupModeChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnSetupModeChanged ( DynExp::ModuleInstance *  Instance, QAction *  Action  ) const

private

Definition at line 1590 of file WidefieldMicroscope.cpp.

OnSpectrumFinishedRecording()

void DynExpModule::Widefield::WidefieldMicroscope::OnSpectrumFinishedRecording ( DynExp::ModuleInstance *  Instance ) const

private

Definition at line 1988 of file WidefieldMicroscope.cpp.

OnStopAction()

void DynExpModule::Widefield::WidefieldMicroscope::OnStopAction ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1499 of file WidefieldMicroscope.cpp.

OnTerminate()

void DynExpModule::Widefield::WidefieldMicroscope::OnTerminate ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1494 of file WidefieldMicroscope.cpp.

OnToggleAutoMeasureConfocalPLEEnabled()

void DynExpModule::Widefield::WidefieldMicroscope::OnToggleAutoMeasureConfocalPLEEnabled ( DynExp::ModuleInstance *  Instance, bool  State  ) const

private

Definition at line 2046 of file WidefieldMicroscope.cpp.

OnToggleAutoMeasureHBTEnabled()

void DynExpModule::Widefield::WidefieldMicroscope::OnToggleAutoMeasureHBTEnabled ( DynExp::ModuleInstance *  Instance, bool  State  ) const

private

Definition at line 2052 of file WidefieldMicroscope.cpp.

OnToggleAutoMeasureOptimizeEnabled()

void DynExpModule::Widefield::WidefieldMicroscope::OnToggleAutoMeasureOptimizeEnabled ( DynExp::ModuleInstance *  Instance, bool  State  ) const

private

Definition at line 2034 of file WidefieldMicroscope.cpp.

OnToggleAutoMeasureSampleRotated()

void DynExpModule::Widefield::WidefieldMicroscope::OnToggleAutoMeasureSampleRotated ( DynExp::ModuleInstance *  Instance, Qt::CheckState  State  ) const

private

Definition at line 2118 of file WidefieldMicroscope.cpp.

OnToggleAutoMeasureSpectrumEnabled()

void DynExpModule::Widefield::WidefieldMicroscope::OnToggleAutoMeasureSpectrumEnabled ( DynExp::ModuleInstance *  Instance, bool  State  ) const

private

Definition at line 2040 of file WidefieldMicroscope.cpp.

OnToggleAutoMeasureWidefieldPLEEnabled()

void DynExpModule::Widefield::WidefieldMicroscope::OnToggleAutoMeasureWidefieldPLEEnabled ( DynExp::ModuleInstance *  Instance, bool  State  ) const

private

Definition at line 2028 of file WidefieldMicroscope.cpp.

OnToggleHBTMirror()

void DynExpModule::Widefield::WidefieldMicroscope::OnToggleHBTMirror ( DynExp::ModuleInstance *  Instance, bool  Checked  ) const

private

Definition at line 1632 of file WidefieldMicroscope.cpp.

OnToggleLEDLightSource()

void DynExpModule::Widefield::WidefieldMicroscope::OnToggleLEDLightSource ( DynExp::ModuleInstance *  Instance, bool  State  ) const

private

Definition at line 1570 of file WidefieldMicroscope.cpp.

OnTogglePumpLightSource()

void DynExpModule::Widefield::WidefieldMicroscope::OnTogglePumpLightSource ( DynExp::ModuleInstance *  Instance, bool  State  ) const

private

Definition at line 1580 of file WidefieldMicroscope.cpp.

OnWidefieldAnalyzeImageDistortion()

void DynExpModule::Widefield::WidefieldMicroscope::OnWidefieldAnalyzeImageDistortion ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1798 of file WidefieldMicroscope.cpp.

OnWidefieldApplyLEDExposureTime()

void DynExpModule::Widefield::WidefieldMicroscope::OnWidefieldApplyLEDExposureTime ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1713 of file WidefieldMicroscope.cpp.

OnWidefieldApplyPumpExposureTime()

void DynExpModule::Widefield::WidefieldMicroscope::OnWidefieldApplyPumpExposureTime ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1730 of file WidefieldMicroscope.cpp.

OnWidefieldFindConfocalSpot()

void DynExpModule::Widefield::WidefieldMicroscope::OnWidefieldFindConfocalSpot ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1741 of file WidefieldMicroscope.cpp.

OnWidefieldImageClicked()

void DynExpModule::Widefield::WidefieldMicroscope::OnWidefieldImageClicked ( DynExp::ModuleInstance *  Instance, QPoint  Position  ) const

private

Definition at line 1818 of file WidefieldMicroscope.cpp.

OnWidefieldLEDExposureTimeChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnWidefieldLEDExposureTimeChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 1707 of file WidefieldMicroscope.cpp.

OnWidefieldLocalizeEmitters()

void DynExpModule::Widefield::WidefieldMicroscope::OnWidefieldLocalizeEmitters ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1808 of file WidefieldMicroscope.cpp.

OnWidefieldPumpExposureTimeChanged()

void DynExpModule::Widefield::WidefieldMicroscope::OnWidefieldPumpExposureTimeChanged ( DynExp::ModuleInstance *  Instance, int  Value  ) const

private

Definition at line 1724 of file WidefieldMicroscope.cpp.

OnWidefieldReadCellID()

void DynExpModule::Widefield::WidefieldMicroscope::OnWidefieldReadCellID ( DynExp::ModuleInstance *  Instance, bool    ) const

private

Definition at line 1788 of file WidefieldMicroscope.cpp.

PLEAcquisitionWaitingStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::PLEAcquisitionWaitingStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2746 of file WidefieldMicroscope.cpp.

PositionPointFromPixelDist()

template<typename PosT >

static ModuleDataType::PositionPoint DynExpModule::Widefield::WidefieldMicroscope::PositionPointFromPixelDist ( Util::SynchronizedPointer< const ParamsType > &  ModuleParams, Util::SynchronizedPointer< ModuleDataType > &  ModuleData, const PosT  x, const PosT  y  )

inlinestaticprivate

Definition at line 603 of file WidefieldMicroscope.h.

PrepareAPDsForConfocalMode()

void DynExpModule::Widefield::WidefieldMicroscope::PrepareAPDsForConfocalMode ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData ) const

private

Definition at line 1072 of file WidefieldMicroscope.cpp.

PrepareImageRecording()

void DynExpModule::Widefield::WidefieldMicroscope::PrepareImageRecording ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData ) const

private

Definition at line 984 of file WidefieldMicroscope.cpp.

RandomPointInCircle()

WidefieldMicroscope::ModuleDataType::PositionPoint DynExpModule::Widefield::WidefieldMicroscope::RandomPointInCircle ( ModuleDataType::PositionType  Radius ) const

private

Returns a uniformly-distributed random coordinate within a circle of radius Radius, not thread-safe.

Definition at line 1220 of file WidefieldMicroscope.cpp.

ReadyStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::ReadyStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2247 of file WidefieldMicroscope.cpp.

RecordImage()

void DynExpModule::Widefield::WidefieldMicroscope::RecordImage ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData ) const

private

Definition at line 998 of file WidefieldMicroscope.cpp.

RegisterConfocalGraphEvents()

void DynExpModule::Widefield::WidefieldMicroscope::RegisterConfocalGraphEvents

(

DynExpQuick::DynExpSurfaceGraphBackend *

ConfocalGraph

)

Definition at line 604 of file WidefieldMicroscope.cpp.

ResetImpl()

void DynExpModule::Widefield::WidefieldMicroscope::ResetImpl ( dispatch_tag< QModuleBase >  )

finaloverrideprivate

Definition at line 685 of file WidefieldMicroscope.cpp.

ResetState()

StateType DynExpModule::Widefield::WidefieldMicroscope::ResetState ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData ) const

private

Aborts a measurement or experimental sequence and continues with the ready state.

Parameters

ModuleData

Reference to WidefieldMicroscope's module data.

Returns

Always returns StateType::Ready.

Definition at line 818 of file WidefieldMicroscope.cpp.

ReturnToReadyStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::ReturnToReadyStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2166 of file WidefieldMicroscope.cpp.

SetFocus()

void DynExpModule::Widefield::WidefieldMicroscope::SetFocus ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData, double  Voltage, bool  IgnoreOffset = false  ) const

private

Definition at line 913 of file WidefieldMicroscope.cpp.

SetHBTSwitch()

void DynExpModule::Widefield::WidefieldMicroscope::SetHBTSwitch ( Util::SynchronizedPointer< const ParamsType > &  ModuleParams, Util::SynchronizedPointer< ModuleDataType > &  ModuleData, bool  IsHBTMode  ) const

private

Definition at line 1105 of file WidefieldMicroscope.cpp.

SetupTransitionBeginStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::SetupTransitionBeginStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2187 of file WidefieldMicroscope.cpp.

SetupTransitionEndStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::SetupTransitionEndStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2224 of file WidefieldMicroscope.cpp.

SetupTransitioningStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::SetupTransitioningStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2219 of file WidefieldMicroscope.cpp.

SpectrumAcquisitionWaitingStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::SpectrumAcquisitionWaitingStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2752 of file WidefieldMicroscope.cpp.

StartAutofocus()

StateType DynExpModule::Widefield::WidefieldMicroscope::StartAutofocus ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData ) const

private

Definition at line 1136 of file WidefieldMicroscope.cpp.

StartAutoMeasureCharacterization()

StateType DynExpModule::Widefield::WidefieldMicroscope::StartAutoMeasureCharacterization ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData, Util::MarkerGraphicsView::MarkerType::IDType  FirstEmitterID = -1  ) const

private

Definition at line 1173 of file WidefieldMicroscope.cpp.

StartAutoMeasureLocalization()

StateType DynExpModule::Widefield::WidefieldMicroscope::StartAutoMeasureLocalization ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData ) const

private

Definition at line 1151 of file WidefieldMicroscope.cpp.

StartAutoMeasureSampleCharacterization()

StateType DynExpModule::Widefield::WidefieldMicroscope::StartAutoMeasureSampleCharacterization ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData ) const

private

Definition at line 1202 of file WidefieldMicroscope.cpp.

StopHBT()

void DynExpModule::Widefield::WidefieldMicroscope::StopHBT ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData ) const

private

Definition at line 1123 of file WidefieldMicroscope.cpp.

UpdatePumpPower()

void DynExpModule::Widefield::WidefieldMicroscope::UpdatePumpPower ( Util::SynchronizedPointer< ModuleDataType > &  ModuleData ) const

private

Definition at line 973 of file WidefieldMicroscope.cpp.

UpdateUIChild()

void DynExpModule::Widefield::WidefieldMicroscope::UpdateUIChild ( const ModuleBase::ModuleDataGetterType &  ModuleDataGetter )

finaloverrideprivate

Definition at line 785 of file WidefieldMicroscope.cpp.

WaitingForImageReadyToCaptureStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::WaitingForImageReadyToCaptureStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2324 of file WidefieldMicroscope.cpp.

WaitingForImageStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::WaitingForImageStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2337 of file WidefieldMicroscope.cpp.

WaitingForWidefieldCellIDStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::WaitingForWidefieldCellIDStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2367 of file WidefieldMicroscope.cpp.

WaitingForWidefieldLocalizationStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::WaitingForWidefieldLocalizationStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2421 of file WidefieldMicroscope.cpp.

WaitingStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::WaitingStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2741 of file WidefieldMicroscope.cpp.

WidefieldCellWaitUntilCenteredStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::WidefieldCellWaitUntilCenteredStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2404 of file WidefieldMicroscope.cpp.

WidefieldImageAcquisitionBeginStateFunc()

StateType DynExpModule::Widefield::WidefieldMicroscope::WidefieldImageAcquisitionBeginStateFunc ( DynExp::ModuleInstance &  Instance )

private

Definition at line 2307 of file WidefieldMicroscope.cpp.

Member Data Documentation

AutofocusBeginState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutofocusBeginState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutofocusBegin,
            &WidefieldMicroscope::AutofocusBeginStateFunc)

Definition at line 804 of file WidefieldMicroscope.h.

AutofocusFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutofocusFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutofocusFinished,
            &WidefieldMicroscope::ReturnToReadyStateFunc)

Definition at line 808 of file WidefieldMicroscope.h.

AutofocusSetupTransitioningContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::AutofocusSetupTransitioningContext

private

Initial value:

= { {
            { StateType::SetupTransitionFinished, StateType::AutofocusBegin }
        }, "Autofocusing..." }

Definition at line 930 of file WidefieldMicroscope.h.

AutofocusWaitingState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutofocusWaitingState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutofocusWaiting,
            &WidefieldMicroscope::AutofocusWaitingStateFunc)

Definition at line 806 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationContext

private

Initial value:

= { {
            { StateType::SetupTransitionFinished, StateType::AutoMeasureCharacterizationStep },
            { StateType::ConfocalOptimizationFinished, StateType::AutoMeasureCharacterizationOptimizationFinished },
            { StateType::SpectrumAcquisitionFinished, StateType::AutoMeasureCharacterizationSpectrumFinished },
            { StateType::PLEAcquisitionFinished, StateType::AutoMeasureCharacterizationPLEFinished },
            { StateType::HBTFinished, StateType::AutoMeasureCharacterizationHBTFinished }
        }, "Characterizing emitters...", { &ConfocalOptimizationContext } }

Definition at line 956 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureCharacterizationFinished,
            &WidefieldMicroscope::ReturnToReadyStateFunc)

Definition at line 910 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationGotoEmitterState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationGotoEmitterState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureCharacterizationGotoEmitter,
            &WidefieldMicroscope::AutoMeasureCharacterizationGotoEmitterStateFunc)

Definition at line 892 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationHBTBeginState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationHBTBeginState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureCharacterizationHBTBegin,
            &WidefieldMicroscope::AutoMeasureCharacterizationHBTBeginStateFunc)

Definition at line 904 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationHBTContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationHBTContext

private

Initial value:

= { {
            { StateType::WaitingFinished, StateType::AutoMeasureCharacterizationHBTBegin }
        }, "Characterizing emitters (HBT acquiring)...", { &AutoMeasureCharacterizationContext } }

Definition at line 972 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationHBTFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationHBTFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureCharacterizationHBTFinished,
            &WidefieldMicroscope::AutoMeasureCharacterizationHBTFinishedStateFunc)

Definition at line 908 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationHBTWaitForInitState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationHBTWaitForInitState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureCharacterizationHBTWaitForInit,
            &WidefieldMicroscope::AutoMeasureCharacterizationHBTWaitForInitStateFunc)

Definition at line 906 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationOptimizationContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationOptimizationContext

private

Initial value:

= { {
            { StateType::WaitingFinished, StateType::ConfocalOptimizationInit }
        }, "Characterizing emitters (optimizing count rate)...", { &AutoMeasureCharacterizationContext } }

Definition at line 963 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationOptimizationFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationOptimizationFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureCharacterizationOptimizationFinished,
            &WidefieldMicroscope::AutoMeasureCharacterizationOptimizationFinishedStateFunc)

Definition at line 894 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationPLEBeginState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationPLEBeginState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureCharacterizationPLEBegin,
            &WidefieldMicroscope::AutoMeasureCharacterizationPLEBeginStateFunc)

Definition at line 900 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationPLEContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationPLEContext

private

Initial value:

= { {
            { StateType::WaitingFinished, StateType::AutoMeasureCharacterizationPLEBegin }
        }, "Characterizing emitters (performing confocal PLE)...", { &AutoMeasureCharacterizationContext } }

Definition at line 969 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationPLEFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationPLEFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureCharacterizationPLEFinished,
            &WidefieldMicroscope::AutoMeasureCharacterizationPLEFinishedStateFunc)

Definition at line 902 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationSpectrumBeginState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationSpectrumBeginState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureCharacterizationSpectrumBegin,
            &WidefieldMicroscope::AutoMeasureCharacterizationSpectrumBeginStateFunc)

Definition at line 896 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationSpectrumContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationSpectrumContext

private

Initial value:

= { {
            { StateType::WaitingFinished, StateType::AutoMeasureCharacterizationSpectrumBegin }
        }, "Characterizing emitters (recording spectrum)...", { &AutoMeasureCharacterizationContext } }

Definition at line 966 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationSpectrumFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationSpectrumFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureCharacterizationSpectrumFinished,
            &WidefieldMicroscope::AutoMeasureCharacterizationSpectrumFinishedStateFunc)

Definition at line 898 of file WidefieldMicroscope.h.

AutoMeasureCharacterizationStepState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureCharacterizationStepState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureCharacterizationStep,
            &WidefieldMicroscope::AutoMeasureCharacterizationStepStateFunc)

Definition at line 890 of file WidefieldMicroscope.h.

AutoMeasureLocalizationContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureLocalizationContext

private

Initial value:

= { {
            { StateType::SetupTransitionFinished, StateType::Waiting },
            { StateType::WaitingFinished, StateType::AutoMeasureLocalizationStep },
            { StateType::WaitingForLEDImageFinished, StateType::AutoMeasureLocalizationSaveLEDImage },
            { StateType::WaitingForWidefieldImageFinished, StateType::AutoMeasureLocalizationSaveWidefieldImage },
            { StateType::PLEAcquisitionFinished, StateType::AutoMeasureLocalizationPLEFinished }
        } }

Definition at line 949 of file WidefieldMicroscope.h.

AutoMeasureLocalizationFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureLocalizationFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureLocalizationFinished,
            &WidefieldMicroscope::ReturnToReadyStateFunc)

Definition at line 888 of file WidefieldMicroscope.h.

AutoMeasureLocalizationMovingState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureLocalizationMovingState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureLocalizationMoving,
            &WidefieldMicroscope::AutoMeasureLocalizationMovingStateFunc, "Moving to next image capturing position...")

Definition at line 882 of file WidefieldMicroscope.h.

AutoMeasureLocalizationPLEBeginState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureLocalizationPLEBeginState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureLocalizationPLEBegin,
            &WidefieldMicroscope::AutoMeasureLocalizationPLEBeginStateFunc)

Definition at line 884 of file WidefieldMicroscope.h.

AutoMeasureLocalizationPLEFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureLocalizationPLEFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureLocalizationPLEFinished,
            &WidefieldMicroscope::AutoMeasureLocalizationPLEFinishedStateFunc)

Definition at line 886 of file WidefieldMicroscope.h.

AutoMeasureLocalizationSaveLEDImageState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureLocalizationSaveLEDImageState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureLocalizationSaveLEDImage,
            &WidefieldMicroscope::AutoMeasureLocalizationSaveLEDImageStateFunc, "Saving LED image...")

Definition at line 878 of file WidefieldMicroscope.h.

AutoMeasureLocalizationSaveWidefieldImageState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureLocalizationSaveWidefieldImageState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureLocalizationSaveWidefieldImage,
            &WidefieldMicroscope::AutoMeasureLocalizationSaveWidefieldImageStateFunc, "Saving widefield image...")

Definition at line 880 of file WidefieldMicroscope.h.

AutoMeasureLocalizationStepState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureLocalizationStepState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureLocalizationStep,
            &WidefieldMicroscope::AutoMeasureLocalizationStepStateFunc, "Recording image...")

Definition at line 876 of file WidefieldMicroscope.h.

AutoMeasureSampleAdvanceCellState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleAdvanceCellState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureSampleAdvanceCell,
            &WidefieldMicroscope::AutoMeasureSampleAdvanceCellStateFunc)

Definition at line 924 of file WidefieldMicroscope.h.

AutoMeasureSampleCharacterizationContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleCharacterizationContext

private

Initial value:

= { {
            { StateType::AutoMeasureCharacterizationFinished, StateType::AutoMeasureSampleAdvanceCell }
        }, "Characterizing sample...", { &AutoMeasureCharacterizationContext } }

Definition at line 990 of file WidefieldMicroscope.h.

AutoMeasureSampleCharacterizationHBTContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleCharacterizationHBTContext

private

Initial value:

= { {
        }, "Characterizing sample (HBT acquiring)...", { &AutoMeasureSampleCharacterizationContext, &AutoMeasureCharacterizationHBTContext } }

Definition at line 999 of file WidefieldMicroscope.h.

AutoMeasureSampleCharacterizationOptimizationContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleCharacterizationOptimizationContext

private

Initial value:

= { {
        }, "Characterizing sample (optimizing count rate)...", { &AutoMeasureSampleCharacterizationContext, &AutoMeasureCharacterizationOptimizationContext } }

Definition at line 993 of file WidefieldMicroscope.h.

AutoMeasureSampleCharacterizationPLEContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleCharacterizationPLEContext

private

Initial value:

= { {
        }, "Characterizing sample (performing confocal PLE)...", { &AutoMeasureSampleCharacterizationContext, &AutoMeasureCharacterizationPLEContext } }

Definition at line 997 of file WidefieldMicroscope.h.

AutoMeasureSampleCharacterizationSpectrumContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleCharacterizationSpectrumContext

private

Initial value:

= { {
        }, "Characterizing sample (recording spectrum)...", { &AutoMeasureSampleCharacterizationContext, &AutoMeasureCharacterizationSpectrumContext } }

Definition at line 995 of file WidefieldMicroscope.h.

AutoMeasureSampleCharacterizeState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleCharacterizeState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureSampleCharacterize,
            &WidefieldMicroscope::AutoMeasureSampleCharacterizeStateFunc)

Definition at line 922 of file WidefieldMicroscope.h.

AutoMeasureSampleContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleContext

private

Initial value:

= { {
            { StateType::AutofocusFinished, StateType::LEDImageAcquisitionBegin },
            { StateType::WaitingForLEDImageReadyToCapture, StateType::Waiting },
            { StateType::WaitingFinished, StateType::WaitingForLEDImageReadyToCapture },
            { StateType::WaitingForLEDImageFinished, StateType::AutoMeasureSampleReadCellID },
            { StateType::WidefieldCellIDReadFinished, StateType::AutoMeasureSampleReadCellIDFinished },
            { StateType::WidefieldLocalizationFinished, StateType::AutoMeasureSampleLocalize }
        }, "Characterizing sample...", { &AutofocusSetupTransitioningContext } }

Definition at line 975 of file WidefieldMicroscope.h.

AutoMeasureSampleFindEmittersState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleFindEmittersState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureSampleFindEmitters,
            &WidefieldMicroscope::AutoMeasureSampleFindEmittersStateFunc)

Definition at line 920 of file WidefieldMicroscope.h.

AutoMeasureSampleFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureSampleFinished,
            &WidefieldMicroscope::ReturnToReadyStateFunc)

Definition at line 926 of file WidefieldMicroscope.h.

AutoMeasureSampleLocalizationContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleLocalizationContext

private

Initial value:

= { {
            { StateType::AutoMeasureLocalizationFinished, StateType::AutoMeasureSampleFindEmitters },
            { StateType::WidefieldLocalizationFinished, StateType::AutoMeasureSampleCharacterize }
        }, "Characterizing sample (localizing)...", { &AutoMeasureLocalizationContext } }

Definition at line 986 of file WidefieldMicroscope.h.

AutoMeasureSampleLocalizeState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleLocalizeState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureSampleLocalize,
            &WidefieldMicroscope::AutoMeasureSampleLocalizeStateFunc)

Definition at line 918 of file WidefieldMicroscope.h.

AutoMeasureSampleReadCellIDFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleReadCellIDFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureSampleReadCellIDFinished,
            &WidefieldMicroscope::AutoMeasureSampleReadCellIDFinishedStateFunc)

Definition at line 916 of file WidefieldMicroscope.h.

AutoMeasureSampleReadCellIDState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleReadCellIDState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureSampleReadCellID,
            &WidefieldMicroscope::AutoMeasureSampleReadCellIDStateFunc)

Definition at line 914 of file WidefieldMicroscope.h.

AutoMeasureSampleRecenterCellContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleRecenterCellContext

private

Initial value:

= { {
            { StateType::WaitingForLEDImageFinished, StateType::AutoMeasureSampleReadCellIDFinished }
        }, "Characterizing sample...", { &AutoMeasureSampleContext } }

Definition at line 983 of file WidefieldMicroscope.h.

AutoMeasureSampleStepState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::AutoMeasureSampleStepState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::AutoMeasureSampleStep,
            &WidefieldMicroscope::AutoMeasureSampleStepStateFunc)

Definition at line 912 of file WidefieldMicroscope.h.

ConfocalOptimizationContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationContext

private

Initial value:

= { {
            { StateType::DummyState, StateType::ConfocalScanStep },
            { StateType::ConfocalScanStep, StateType::ConfocalOptimizationStep }
        }, "Optimizing count rate..." }

Definition at line 939 of file WidefieldMicroscope.h.

ConfocalOptimizationFeedbackFuture

std::future<ConfocalOptimizationFeedbackType> DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationFeedbackFuture

private

Definition at line 1028 of file WidefieldMicroscope.h.

ConfocalOptimizationFeedbackPromise

std::promise<ConfocalOptimizationFeedbackType> DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationFeedbackPromise

mutableprivate

Definition at line 1027 of file WidefieldMicroscope.h.

ConfocalOptimizationFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::ConfocalOptimizationFinished,
            &WidefieldMicroscope::ReturnToReadyStateFunc)

Definition at line 858 of file WidefieldMicroscope.h.

ConfocalOptimizationInitState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationInitState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::ConfocalOptimizationInit,
            &WidefieldMicroscope::ConfocalOptimizationInitStateFunc)

Definition at line 850 of file WidefieldMicroscope.h.

ConfocalOptimizationInitSubStepState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationInitSubStepState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::ConfocalOptimizationInitSubStep,
            &WidefieldMicroscope::ConfocalOptimizationInitSubStepStateFunc)

Definition at line 852 of file WidefieldMicroscope.h.

ConfocalOptimizationNumStepsPerformed

std::atomic<size_t> DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationNumStepsPerformed = 0

mutableprivate

Definition at line 1023 of file WidefieldMicroscope.h.

ConfocalOptimizationPromisesRenewed

std::atomic_bool DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationPromisesRenewed = false

mutableprivate

Definition at line 1024 of file WidefieldMicroscope.h.

ConfocalOptimizationStateFuture

std::future<ConfocalOptimizationStateType> DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationStateFuture

private

Definition at line 1026 of file WidefieldMicroscope.h.

ConfocalOptimizationStatePromise

std::promise<ConfocalOptimizationStateType> DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationStatePromise

private

Definition at line 1025 of file WidefieldMicroscope.h.

ConfocalOptimizationStepState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationStepState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::ConfocalOptimizationStep,
            &WidefieldMicroscope::ConfocalOptimizationStepStateFunc)

Definition at line 856 of file WidefieldMicroscope.h.

ConfocalOptimizationThreadReturnFuture

std::future<ConfocalOptimizationThreadReturnType> DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationThreadReturnFuture

private

Definition at line 1029 of file WidefieldMicroscope.h.

ConfocalOptimizationWaitState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::ConfocalOptimizationWaitState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::ConfocalOptimizationWait,
            &WidefieldMicroscope::ConfocalOptimizationWaitStateFunc)

Definition at line 854 of file WidefieldMicroscope.h.

ConfocalScanCaptureState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::ConfocalScanCaptureState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::ConfocalScanCapture,
            &WidefieldMicroscope::ConfocalScanCaptureStateFunc, "Performing confocal scan...")

Definition at line 846 of file WidefieldMicroscope.h.

ConfocalScanPositionerStateX

const std::shared_ptr<AtomicPositionerStateType> DynExpModule::Widefield::WidefieldMicroscope::ConfocalScanPositionerStateX

private

Definition at line 1006 of file WidefieldMicroscope.h.

ConfocalScanPositionerStateY

const std::shared_ptr<AtomicPositionerStateType> DynExpModule::Widefield::WidefieldMicroscope::ConfocalScanPositionerStateY

private

Definition at line 1007 of file WidefieldMicroscope.h.

ConfocalScanPositionerStateZ

const std::shared_ptr<AtomicPositionerStateType> DynExpModule::Widefield::WidefieldMicroscope::ConfocalScanPositionerStateZ

private

Definition at line 1008 of file WidefieldMicroscope.h.

ConfocalScanPositions

std::list<WidefieldMicroscopeData::PositionPoint> DynExpModule::Widefield::WidefieldMicroscope::ConfocalScanPositions

mutableprivate

Definition at line 1014 of file WidefieldMicroscope.h.

ConfocalScanStepState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::ConfocalScanStepState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::ConfocalScanStep,
            &WidefieldMicroscope::ConfocalScanStepStateFunc, "Performing confocal scan...")

Definition at line 842 of file WidefieldMicroscope.h.

ConfocalScanWaitUntilCapturedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::ConfocalScanWaitUntilCapturedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::ConfocalScanWaitUntilCaptured,
            &WidefieldMicroscope::ConfocalScanWaitUntilCapturedStateFunc, "Performing confocal scan...")

Definition at line 848 of file WidefieldMicroscope.h.

ConfocalScanWaitUntilMovedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::ConfocalScanWaitUntilMovedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::ConfocalScanWaitUntilMoved,
            &WidefieldMicroscope::ConfocalScanWaitUntilMovedStateFunc, "Performing confocal scan...")

Definition at line 844 of file WidefieldMicroscope.h.

FindingConfocalSpotAfterRecordingWidefieldImageState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::FindingConfocalSpotAfterRecordingWidefieldImageState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::FindingConfocalSpotAfterRecordingWidefieldImage,
            &WidefieldMicroscope::FindingConfocalSpotAfterRecordingWidefieldImageStateFunc)

Definition at line 840 of file WidefieldMicroscope.h.

FindingConfocalSpotAfterTransitioningToConfocalModeState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::FindingConfocalSpotAfterTransitioningToConfocalModeState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::FindingConfocalSpotAfterTransitioningToConfocalMode,
            &WidefieldMicroscope::FindingConfocalSpotAfterTransitioningToConfocalModeStateFunc)

Definition at line 838 of file WidefieldMicroscope.h.

FindingConfocalSpotBeginContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::FindingConfocalSpotBeginContext

private

Initial value:

= { {
            { StateType::SetupTransitionFinished, StateType::FindingConfocalSpotAfterTransitioningToConfocalMode }
        }, "Locating confocal spot..." }

Definition at line 933 of file WidefieldMicroscope.h.

FindingConfocalSpotBeginState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::FindingConfocalSpotBeginState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::FindingConfocalSpotBegin,
            &WidefieldMicroscope::FindingConfocalSpotBeginStateFunc)

Definition at line 836 of file WidefieldMicroscope.h.

FindingConfocalSpotRecordingWidefieldImageContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::FindingConfocalSpotRecordingWidefieldImageContext

private

Initial value:

= { {
            { StateType::WaitingForWidefieldImageFinished, StateType::FindingConfocalSpotAfterRecordingWidefieldImage }
        }, "Locating confocal spot..." }

Definition at line 936 of file WidefieldMicroscope.h.

GSLConfocalOptimizationFuncDesc

gsl_multimin_function DynExpModule::Widefield::WidefieldMicroscope::GSLConfocalOptimizationFuncDesc { &WidefieldMicroscope::ConfocalOptimizationFuncForwarder, GSLConfocalOptimizationNumDimensions, this }

private

Definition at line 1019 of file WidefieldMicroscope.h.

GSLConfocalOptimizationInitialPoint

gsl_vector* const DynExpModule::Widefield::WidefieldMicroscope::GSLConfocalOptimizationInitialPoint

private

Definition at line 1022 of file WidefieldMicroscope.h.

GSLConfocalOptimizationMinimizer

const gsl_multimin_fminimizer_type* const DynExpModule::Widefield::WidefieldMicroscope::GSLConfocalOptimizationMinimizer = gsl_multimin_fminimizer_nmsimplex2

private

Definition at line 1018 of file WidefieldMicroscope.h.

GSLConfocalOptimizationNumDimensions

constexpr size_t DynExpModule::Widefield::WidefieldMicroscope::GSLConfocalOptimizationNumDimensions = 3

staticconstexprprivate

Definition at line 1017 of file WidefieldMicroscope.h.

GSLConfocalOptimizationState

gsl_multimin_fminimizer* const DynExpModule::Widefield::WidefieldMicroscope::GSLConfocalOptimizationState

private

Definition at line 1020 of file WidefieldMicroscope.h.

GSLConfocalOptimizationStepSize

gsl_vector* const DynExpModule::Widefield::WidefieldMicroscope::GSLConfocalOptimizationStepSize

private

Definition at line 1021 of file WidefieldMicroscope.h.

HBTAcquiringState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::HBTAcquiringState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::HBTAcquiring,
            &WidefieldMicroscope::HBTAcquiringStateFunc, "HBT acquiring...")

Definition at line 860 of file WidefieldMicroscope.h.

HBTFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::HBTFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::HBTFinished,
            &WidefieldMicroscope::ReturnToReadyStateFunc)

Definition at line 862 of file WidefieldMicroscope.h.

HBTIntegrationTimeBeforeReset

std::chrono::microseconds DynExpModule::Widefield::WidefieldMicroscope::HBTIntegrationTimeBeforeReset { 0 }

mutableprivate

Definition at line 1040 of file WidefieldMicroscope.h.

ImageCapturingPaused

bool DynExpModule::Widefield::WidefieldMicroscope::ImageCapturingPaused = false

mutableprivate

Definition at line 1036 of file WidefieldMicroscope.h.

InitializingState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::InitializingState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::Initializing,
            &WidefieldMicroscope::InitializingStateFunc, "Initializing module...")

Definition at line 792 of file WidefieldMicroscope.h.

LEDImageAcquisitionBeginState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::LEDImageAcquisitionBeginState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::LEDImageAcquisitionBegin,
            &WidefieldMicroscope::LEDImageAcquisitionBeginStateFunc, "Recording LED image...")

Definition at line 810 of file WidefieldMicroscope.h.

LEDImageAcquisitionSetupTransitioningContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::LEDImageAcquisitionSetupTransitioningContext

private

Initial value:

= { {
            { StateType::SetupTransitionFinished, StateType::LEDImageAcquisitionBegin }
        } }

Definition at line 943 of file WidefieldMicroscope.h.

LogUIMessagesOnly

bool DynExpModule::Widefield::WidefieldMicroscope::LogUIMessagesOnly = false

mutableprivate

Definition at line 1044 of file WidefieldMicroscope.h.

NumFailedUpdateAttempts

size_t DynExpModule::Widefield::WidefieldMicroscope::NumFailedUpdateAttempts = 0

private

Definition at line 1043 of file WidefieldMicroscope.h.

PLEAcquisitionFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::PLEAcquisitionFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::PLEAcquisitionFinished,
            &WidefieldMicroscope::ReturnToReadyStateFunc)

Definition at line 870 of file WidefieldMicroscope.h.

PLEAcquisitionWaitingState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::PLEAcquisitionWaitingState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::PLEAcquisitionWaiting,
            &WidefieldMicroscope::PLEAcquisitionWaitingStateFunc, "Performing widefield PLE...")

Definition at line 868 of file WidefieldMicroscope.h.

ReadyState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::ReadyState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::Ready,
            &WidefieldMicroscope::ReadyStateFunc, "Ready")

Definition at line 802 of file WidefieldMicroscope.h.

SetupTransitionBeginState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::SetupTransitionBeginState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::SetupTransitionBegin,
            &WidefieldMicroscope::SetupTransitionBeginStateFunc, "Transitioning...")

Definition at line 794 of file WidefieldMicroscope.h.

SetupTransitionEndState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::SetupTransitionEndState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::SetupTransitionEnd,
            &WidefieldMicroscope::SetupTransitionEndStateFunc, "Transitioning...")

Definition at line 798 of file WidefieldMicroscope.h.

SetupTransitionFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::SetupTransitionFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::SetupTransitionFinished,
            &WidefieldMicroscope::ReturnToReadyStateFunc)

Definition at line 800 of file WidefieldMicroscope.h.

SetupTransitionFinishedTimePoint

std::chrono::system_clock::time_point DynExpModule::Widefield::WidefieldMicroscope::SetupTransitionFinishedTimePoint

private

Definition at line 1032 of file WidefieldMicroscope.h.

SetupTransitioningState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::SetupTransitioningState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::SetupTransitioning,
            &WidefieldMicroscope::SetupTransitioningStateFunc, "Transitioning...")

Definition at line 796 of file WidefieldMicroscope.h.

SpectrumAcquisitionFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::SpectrumAcquisitionFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::SpectrumAcquisitionFinished,
            &WidefieldMicroscope::ReturnToReadyStateFunc)

Definition at line 874 of file WidefieldMicroscope.h.

SpectrumAcquisitionWaitingState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::SpectrumAcquisitionWaitingState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::SpectrumAcquisitionWaiting,
            &WidefieldMicroscope::SpectrumAcquisitionWaitingStateFunc, "Acquiring spectrum...")

Definition at line 872 of file WidefieldMicroscope.h.

StateMachine

Util::StateMachine<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::StateMachine

mutableprivate

Definition at line 1003 of file WidefieldMicroscope.h.

TurnOnPumpSourceAfterTransitioning

bool DynExpModule::Widefield::WidefieldMicroscope::TurnOnPumpSourceAfterTransitioning = false

private

Definition at line 1033 of file WidefieldMicroscope.h.

WaitingEndTimePoint

std::chrono::system_clock::time_point DynExpModule::Widefield::WidefieldMicroscope::WaitingEndTimePoint

mutableprivate

Definition at line 1039 of file WidefieldMicroscope.h.

WaitingFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::WaitingFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::WaitingFinished,
            &WidefieldMicroscope::ReturnToReadyStateFunc)

Definition at line 866 of file WidefieldMicroscope.h.

WaitingForLEDImageFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::WaitingForLEDImageFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::WaitingForLEDImageFinished,
            &WidefieldMicroscope::ReturnToReadyStateFunc)

Definition at line 818 of file WidefieldMicroscope.h.

WaitingForLEDImageReadyToCaptureState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::WaitingForLEDImageReadyToCaptureState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::WaitingForLEDImageReadyToCapture,
            &WidefieldMicroscope::WaitingForImageReadyToCaptureStateFunc, "Recording LED image...")

Definition at line 814 of file WidefieldMicroscope.h.

WaitingForLEDImageState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::WaitingForLEDImageState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::WaitingForLEDImage,
            &WidefieldMicroscope::WaitingForImageStateFunc, "Recording LED image...")

Definition at line 816 of file WidefieldMicroscope.h.

WaitingForWidefieldCellIDState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::WaitingForWidefieldCellIDState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::WaitingForWidefieldCellID,
            &WidefieldMicroscope::WaitingForWidefieldCellIDStateFunc, "Waiting for cell ID to be read from image...")

Definition at line 826 of file WidefieldMicroscope.h.

WaitingForWidefieldImageFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::WaitingForWidefieldImageFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::WaitingForWidefieldImageFinished,
            &WidefieldMicroscope::ReturnToReadyStateFunc)

Definition at line 824 of file WidefieldMicroscope.h.

WaitingForWidefieldImageReadyToCaptureState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::WaitingForWidefieldImageReadyToCaptureState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::WaitingForWidefieldImageReadyToCapture,
            &WidefieldMicroscope::WaitingForImageReadyToCaptureStateFunc, "Recording widefield image...")

Definition at line 820 of file WidefieldMicroscope.h.

WaitingForWidefieldImageState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::WaitingForWidefieldImageState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::WaitingForWidefieldImage,
            &WidefieldMicroscope::WaitingForImageStateFunc, "Recording widefield image...")

Definition at line 822 of file WidefieldMicroscope.h.

WaitingForWidefieldLocalizationState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::WaitingForWidefieldLocalizationState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::WaitingForWidefieldLocalization,
            &WidefieldMicroscope::WaitingForWidefieldLocalizationStateFunc, "Waiting for localization of emitters from image...")

Definition at line 832 of file WidefieldMicroscope.h.

WaitingState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::WaitingState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::Waiting,
            &WidefieldMicroscope::WaitingStateFunc, "Waiting...")

Definition at line 864 of file WidefieldMicroscope.h.

WidefieldCellIDReadFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::WidefieldCellIDReadFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::WidefieldCellIDReadFinished,
            &WidefieldMicroscope::ReturnToReadyStateFunc)

Definition at line 830 of file WidefieldMicroscope.h.

WidefieldCellIDState

const std::shared_ptr<AtomicWidefieldImageProcessingStateType> DynExpModule::Widefield::WidefieldMicroscope::WidefieldCellIDState

private

Definition at line 1011 of file WidefieldMicroscope.h.

WidefieldCellWaitUntilCenteredState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::WidefieldCellWaitUntilCenteredState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::WidefieldCellWaitUntilCentered,
            &WidefieldMicroscope::WidefieldCellWaitUntilCenteredStateFunc, "Waiting for cell to be centered in FOV...")

Definition at line 828 of file WidefieldMicroscope.h.

WidefieldImageAcquisitionBeginState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::WidefieldImageAcquisitionBeginState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::WidefieldImageAcquisitionBegin,
            &WidefieldMicroscope::WidefieldImageAcquisitionBeginStateFunc, "Recording widefield image...")

Definition at line 812 of file WidefieldMicroscope.h.

WidefieldImageAcquisitionSetupTransitioningContext

const Util::StateMachineContext<StateMachineStateType> DynExpModule::Widefield::WidefieldMicroscope::WidefieldImageAcquisitionSetupTransitioningContext

private

Initial value:

= { {
            { StateType::SetupTransitionFinished, StateType::WidefieldImageAcquisitionBegin }
        } }

Definition at line 946 of file WidefieldMicroscope.h.

WidefieldLocalizationFinishedState

constexpr auto DynExpModule::Widefield::WidefieldMicroscope::WidefieldLocalizationFinishedState

staticconstexprprivate

Initial value:

= Util::StateMachineState(StateType::WidefieldLocalizationFinished,
            &WidefieldMicroscope::ReturnToReadyStateFunc)

Definition at line 834 of file WidefieldMicroscope.h.

WidefieldLocalizationState

const std::shared_ptr<AtomicWidefieldImageProcessingStateType> DynExpModule::Widefield::WidefieldMicroscope::WidefieldLocalizationState

private

Definition at line 1012 of file WidefieldMicroscope.h.

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The documentation for this class was generated from the following files:

• WidefieldMicroscope.h
• WidefieldMicroscope.cpp